http://www.cnr.it/ontology/cnr/individuo/prodotto/ID37705
Mitochondrial thioredoxin reductase inhibition by gold (I) compounds and concurrent stimulation of permeability transition and release of cytochrome c (Articolo in rivista)
- Type
- Label
- Mitochondrial thioredoxin reductase inhibition by gold (I) compounds and concurrent stimulation of permeability transition and release of cytochrome c (Articolo in rivista) (literal)
- Anno
- 2004-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1016/j.bcp.2003.09.038 (literal)
- Alternative label
Rigobello MP, Scutari G, Folda A, Bindoli A (2004)
Mitochondrial thioredoxin reductase inhibition by gold (I) compounds and concurrent stimulation of permeability transition and release of cytochrome c
in Biochemical pharmacology
(literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Rigobello MP, Scutari G, Folda A, Bindoli A (literal)
- Pagina inizio
- Pagina fine
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
- Rivista
- Note
- ISI Web of Science (WOS) (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- Dipartimento di Chimica Biologica, Universita` di Padova, Viale G. Colombo 3, 35121 Padua, Italy
Istituto di Neuroscienze, Sezione di Biomembrane (CNR), Dipartimento di Chimica Biologica, Viale G. Colombo 3, 35121 Padua, Italy (literal)
- Titolo
- Mitochondrial thioredoxin reductase inhibition by gold (I) compounds and concurrent stimulation of permeability transition and release of cytochrome c (literal)
- Abstract
- The effects of auranofin, chloro(triethylphosphine)gold(I) (TEPAu), and aurothiomalate on mitochondrial respiration, pyridine
nucleotide redox state, membrane permeability properties, and redox enzymes activities were compared. The three gold(I) derivatives,
in the submicromolar range, were extremely potent inhibitors of thioredoxin reductase and stimulators of the mitochondrial membrane
permeability transition (MPT). Auranofin appeared as the most effective one. In the micromolar range, it inhibited respiratory chain and
glutathione peroxidase activity only slightly if not at all. TEPAu and aurothiomalate exhibited effects similar to auranofin, although
TEPAu showed a moderate inhibition on respiration. Aurothiomalate inhibited glutathione peroxidase at concentrations where auranofin
and TEPAu were without effect. Under nonswelling conditions, the presence of auranofin and aurothiomalate did not alter the redox
properties of the mitochondrial pyridine nucleotides indicating that membrane permeability transition occurred independently of the
preliminary oxidation of pyridine nucleotides. Under the same experimental conditions, TEPAu showed a moderate stimulation of
pyridine nucleotides oxidation. Mitochondrial total thiol groups, in the presence of the gold(I) derivatives, slightly decreased, indicating
the occurrence of an oxidative trend. Concomitantly with MPT, gold(I) compounds determined the release of cytochrome c that, however,
occurred also in the presence of cyclosporin A and, partially, of EGTA, indicating its independence of MPT. It is concluded that the
specific inhibition of thioredoxin reductase by gold(I) compounds may be the determinant of MPT and the release of cytochrome c. (literal)
- Prodotto di
- Autore CNR
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