Calpain activity contributes to the control of SNAP-25 levels in neurons. (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Calpain activity contributes to the control of SNAP-25 levels in neurons. (Articolo in rivista) (literal)

Anno

• 2008-01-01T00:00:00+01:00 (literal)

Alternative label

• Grumelli C, Berghuis P, Pozzi D, Caleo M, Antonucci F, Bonanno G, Carmignoto G, Dobszay MB, Harkany T, Matteoli M, Verderio C (2008)
  Calpain activity contributes to the control of SNAP-25 levels in neurons.
  in Molecular and cellular neurosciences (Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Grumelli C, Berghuis P, Pozzi D, Caleo M, Antonucci F, Bonanno G, Carmignoto G, Dobszay MB, Harkany T, Matteoli M, Verderio C (literal)

Pagina inizio

• 314 (literal)

Pagina fine

• 323 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 39 (literal)

Rivista

• Molecular and cellular neurosciences (Rivista)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• 1. Univ Milan, Dipartimento Farmacol Med, Ist CNR Neurosci, I-20122 Milan, Italy 2. Karolinska Inst, Dept Med Biochem & Biophys, Div Mol Neurobiol, Stockholm, Sweden 3. Ist CNR Neurosci, Pisa, Italy 4. Univ Genoa, Ctr Excellence Biomed Res, Dept Expt Med, Genoa, Italy 5. Univ Padua, Dipartimento Sci Biomed Sperimentali, Ist CNR Neurosci, I-35100 Padua, Italy 6. Univ Aberdeen, Coll Life Sci & Med, Inst Med Sci, Aberdeen AB9 1FX, Scotland 7. IRCCS Fdn Don Gnocchi, Milan, Italy (literal)

Titolo

• Calpain activity contributes to the control of SNAP-25 levels in neurons. (literal)

Abstract

• Calpains are a family of calcium-dependent proteases with abundant expression in the CNS, and potent in cleaving some synaptic components. Assessment of calpain activity by its fluorescent substrate, Boc-Leu-Met-CMAC, revealed that Cultured neurons display a significant level of constitutive enzyme activity. Notably, calpain activity differs in distinct neuronal populations, with a significantly higher level of activity in GABAergic cells. Using selectively-enriched cultures of fast-spiking GABAergic interneurons, we show that calpain activity partially contributes to the post-translational down regulation of SNAP-25, a calpain substrate, in differentiated GABA cells. In addition, we demonstrate that SNAP-25 is cleaved by calpain in response to acute seizures induced by intraperitoneal kainate injection in vivo. These data indicate that calpains in neurons are active even at physiological calcium concentrations and that different levels of calpain activation in selected neuron subtypes may contribute to the pattern of synaptic protein expression (literal)

Prodotto di

• Neurobiologia e Neuropatologia (ME.P02.005.001) (Modulo)
• Istituto di neuroscienze (IN) (Istituto)
• Farmacologia Cellulare e Molecolare delle Cellule Nervose (ME.P02.007.001) (Modulo)

Autore CNR

• PIERGIORGIO CARMIGNOTO (Unità di personale interno)
• DAVIDE POZZI (Unità di personale esterno)
• MICHELA MATTEOLI (Persona)
• FLAVIA ANTONUCCI (Unità di personale esterno)
• MATTEO CALEO (Unità di personale interno)
• CLAUDIA VERDERIO (Unità di personale interno)

Incoming links:

Autore CNR di

• MATTEO CALEO (Unità di personale interno)
• PIERGIORGIO CARMIGNOTO (Unità di personale interno)
• CLAUDIA VERDERIO (Unità di personale interno)
• MICHELA MATTEOLI (Persona)
• FLAVIA ANTONUCCI (Unità di personale esterno)
• DAVIDE POZZI (Unità di personale esterno)

Prodotto

• Istituto di neuroscienze (IN) (Istituto)
• Farmacologia Cellulare e Molecolare delle Cellule Nervose (ME.P02.007.001) (Modulo)
• Neurobiologia e Neuropatologia (ME.P02.005.001) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Molecular and cellular neurosciences (Rivista)