http://www.cnr.it/ontology/cnr/individuo/prodotto/ID37419
Role of mitochondria in serum withdrawal-induced apoptosis of immortalized neuronal precursors (Articolo in rivista)
- Type
- Label
- Role of mitochondria in serum withdrawal-induced apoptosis of immortalized neuronal precursors (Articolo in rivista) (literal)
- Anno
- 2002-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1016/S0165-3806(01)00326-1 (literal)
- Alternative label
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Colombaioni L; Colombini L; Garcia-Gil M. (literal)
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- Rivista
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali
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- ISI Web of Science (WOS) (literal)
- Google Scholar (literal)
- PubMed (literal)
- Scopus (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- Istituto di Neuroscienze, Pisa , Italy
Università di Pisa, Dipartimento di Fisiologia e Biochimica, Pisa, Italy (literal)
- Titolo
- Role of mitochondria in serum withdrawal-induced apoptosis of immortalized neuronal precursors (literal)
- Abstract
- The intracellular mechanisms controlling apoptosis in immature neurons are
still largely unknown. Taking immortalized hippocampal neuronal precursors
(mouse cell line HN9.10e) as a model, we have analyzed the cellular events
associated to apoptosis induced by serum deprivation. We observed
translocation of Bax from cytosol to mitochondria after 1 h of serum
withdrawal followed, 2 h later, by cytochrome c release from mitochondria.
These events occurred without mitochondrial membrane potential loss nor
mitochondrial calcium raise. As calcium is implicated in several cell
death pathways, we analyzed intracellular calcium levels after longer
periods of serum deprivation. After 6 h, an increase of cytosolic Ca2+ was
detected in HN9.10e cells loaded with the Ca2+ indicator Fluo3-AM. This
increase of calcium preceded morphological signs of apoptosis such as cell
shrinkage and nuclear fragmentation, and was followed by a more pronounced
raise that persisted until the terminal phases of the apoptotic process.
Cells serum-deprived for 4 h and then grown in complete medium for 20 h
fully recovered viability. Summarizing, in HN9.10e cells, calcium
deregulation occurs in the late phases of apoptosis; earlier events
involve translocation of Bax, release of cytochrome c, and maintenance of
mitochondrial functionality. This allows an enlargement of the temporal
window in which commitment to death is reversible. (literal)
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