Serum deprivation increases ceramide levels and induces apoptosis in undifferentiated HN9.10e cells (Articolo in rivista)

Type
Label
  • Serum deprivation increases ceramide levels and induces apoptosis in undifferentiated HN9.10e cells (Articolo in rivista) (literal)
Anno
  • 2002-01-01T00:00:00+01:00 (literal)
Alternative label
  • Colombaioni L.*Frago L. M.*Varela-Nieto I., Pesi R.* Garcia-Gil M. (2002)
    Serum deprivation increases ceramide levels and induces apoptosis in undifferentiated HN9.10e cells
    in Neurochemistry international; Pergamon Press, Oxford (Regno Unito)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Colombaioni L.*Frago L. M.*Varela-Nieto I., Pesi R.* Garcia-Gil M. (literal)
Pagina inizio
  • 327 (literal)
Pagina fine
  • 336 (literal)
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  • 40 (literal)
Rivista
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  • 10 (literal)
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  • 4 (literal)
Note
  • ISI Web of Science (WOS) (literal)
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  • Istituto di Neuroscienze, I-56100 Pisa, Italy Universit√† di Pisa, Dipartimentodi fisiologia e Biochimica, I-56127 Pisa, Italy UAM, CSIC, Inst. Invest. Biomed. Alberto Sols, Madrid 28029, Spain (literal)
Titolo
  • Serum deprivation increases ceramide levels and induces apoptosis in undifferentiated HN9.10e cells (literal)
Abstract
  • Sphingolipid metabolites have been involved in the regulation of proliferation, differentiation and apoptosis. While cellular mechanisms of these processes have been extensively analysed in the post-mitotic neurons, little is known about proliferating neuronal precursors. We have taken as a model of neuroblasts the embryonic hippocampal cell line HN9.10e. Apoptosis was induced by serum deprivation and by treatment with N-acetylsphingosine (C2-Cer), a membrane-permeant analogue of the second messenger ceramide. Following C2-Cer addition, cytochrome c was released from mitochondria, [Ca(2+)](i) and caspase-3-like activity increased. Both cytochrome c release and rise of [Ca(2+)](i) occurred before caspase-3 activation and nuclear condensation. The intracellular levels of ceramide peaked at 1h following the serum deprivation. These results indicate that the serum deprivation induces a rise in the intracellular ceramide level, and that increased ceramide concentration leads to calcium dysregulation and release of cytochrome c followed by caspase-3 activation. We show that cytochrome c is released without a loss of mitochondrial transmembrane potential. (literal)
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