http://www.cnr.it/ontology/cnr/individuo/prodotto/ID37388
Erythropoietin exerts an anti-inflammatory effect on the CNS in a model of experimental autoimmune encephalomyelitis. (Articolo in rivista)
- Type
- Label
- Erythropoietin exerts an anti-inflammatory effect on the CNS in a model of experimental autoimmune encephalomyelitis. (Articolo in rivista) (literal)
- Anno
- 2002-01-01T00:00:00+01:00 (literal)
- Alternative label
Agnello D., Bigini P., Villa P., Mennini T., Cerami A., Brines M.L., Ghezzi P. (2002)
Erythropoietin exerts an anti-inflammatory effect on the CNS in a model of experimental autoimmune encephalomyelitis.
in Brain research
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- Agnello D., Bigini P., Villa P., Mennini T., Cerami A., Brines M.L., Ghezzi P. (literal)
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- ISI Web of Science (WOS) (literal)
- Titolo
- Erythropoietin exerts an anti-inflammatory effect on the CNS in a model of experimental autoimmune encephalomyelitis. (literal)
- Abstract
- In recent work we reported that systemically administered
erythropoietin (EPO) crosses the blood-brain barrier and has
protective effects in animal models of cerebral ischemia, brain
trauma and in a rat model of experimental autoimmune
encephalomyelitis (EAE). Here we characterize the effect of systemic
EPO on the inflammatory component of actively induced, acute EAE in
Lewis rats. Administration of EPO at doses of 500-5000 U/kg bw i.p.,
daily from day 3 after immunization with myelin basic protein (MBP),
delayed the onset of EAE and decreased its clinical score at peak
time (days 12-13). Immunohistochemical analysis of the spinal cord
using anti-glial fibrillary acidic protein (GFAP) and anti-CD11b
antibodies showed that EPO markedly diminished inflammation and
glial activation/proliferation. EAE induced significant levels of TNF and
IL-6 in the spinal cord, where IL-6 was maximum at the onset of the
disease (day 10) and TNF at its peak (day 12). EPO delayed the
increase of TNF levels, without altering their peak levels, and
markedly reduced those of IL-6 suggesting that the decreased
inflammation and clinical score may be in part upon attenuation of
IL-6. On the other hand, EPO was without effect in a model of
adjuvant-induced arthritis in Lewis rats, suggesting a specificity
towards autoimmune demyelinating diseases. These data suggest
that EPO might act as a protective cytokine in inflammatory
pathologies of the CNS.
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