Differentiation of human adult CD34+ stem cells into cells wih a neural phenotype: role of astrocytes (Articolo in rivista)

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  • Differentiation of human adult CD34+ stem cells into cells wih a neural phenotype: role of astrocytes (Articolo in rivista) (literal)
Anno
  • 2006-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1016/j.expneurol.2005.10.004 (literal)
Alternative label
  • Reali C, Scintu F; Pillai R; Cabras S; Argiolu F; Ristaldi MS; Sanna MA; Badiali m; Sogos V. (2006)
    Differentiation of human adult CD34+ stem cells into cells wih a neural phenotype: role of astrocytes
    in Experimental neurology
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Reali C, Scintu F; Pillai R; Cabras S; Argiolu F; Ristaldi MS; Sanna MA; Badiali m; Sogos V. (literal)
Pagina inizio
  • 399 (literal)
Pagina fine
  • 406 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 197 (literal)
Rivista
Note
  • ISI Web of Science (WOS) (literal)
  • PubMe (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Ristaldi MS; IRGB-CNR Reali C, Scintu F, Pillai R, Cabras S, Sogos V: Depatment of Cytoporphology, Cagliari University Argiolu F, Badiali M: \"Ospedale Regionale per le Microcitemie\", Cagliari (literal)
Titolo
  • Differentiation of human adult CD34+ stem cells into cells wih a neural phenotype: role of astrocytes (literal)
Abstract
  • It has recently been reported that adult hematopoietic stem cells can differentiate into neural cells, opening new frontiers in therapy for neurodegenerative diseases. In this study, adult human hematopoietic stein cells (HSCs) were isolated via magnetic bead sorting, using a specific CD34 antibody and cultured with human astrocyte Culture conditioned medium (ACM). In order to evaluate their differentiation into neurons and/or astrocytes, ACM-treated cultures were probed for the expression of several neural markers. We observed morphological modifications and, after 20 days of treatment, cell morphology displayed extending processes. Immunocytochemistry, Western blotting and RTPCR showed the expression of neuronal markers such as neurofilaments, neuron specific enolase (NSE) and NeuN in ACM-treated HSCs cultured in poly-L-lysine-coated dishes. On the contrary, when the same ACM-treated cells were grown on a plastic substrate, they expressed high levels of glial fibrillary acidic protein (GFAP), with only weak expression of neuronal markers. Nestin, a neural progenitor cell marker, was present in treated cells, regardless of the Substrate. These results demonstrate that astrocytes can generate a suitable microenvironment for inducing HSCs to differentiate into neural cells. Therefore, adult bone marrow may represent a readily accessible source of cells for treating neurodegenerative diseases. (c) 2005 Elsevier Inc. All rights reserved (literal)
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