http://www.cnr.it/ontology/cnr/individuo/prodotto/ID37164
Nuclear factor-erythroid 2 (NF-E2) expression in normal and malignant megakaryocytopoies (Articolo in rivista)
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- Nuclear factor-erythroid 2 (NF-E2) expression in normal and malignant megakaryocytopoies (Articolo in rivista) (literal)
- Anno
- 2002-01-01T00:00:00+01:00 (literal)
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Catani L., Vianelli N., Amabile M., Pattacini L., Valdre L., Fagioli M. E., Poli M., Gugliotta L., Moi P., Marini M. G., Martinelli G., Tura S., Baccarani M. (2002)
Nuclear factor-erythroid 2 (NF-E2) expression in normal and malignant megakaryocytopoies
in Leukemia
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- Catani L., Vianelli N., Amabile M., Pattacini L., Valdre L., Fagioli M. E., Poli M., Gugliotta L., Moi P., Marini M. G., Martinelli G., Tura S., Baccarani M. (literal)
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- Titolo
- Nuclear factor-erythroid 2 (NF-E2) expression in normal and malignant megakaryocytopoies (literal)
- Abstract
- Although the transcription factor nuclear factor-erythroid 2 (NFE2)
is known to be functionally linked to the megakaryocytic
lineage, little is known about its role in malignant megakaryocytes.
We used real-time RT-PCR and Western blotting to
investigate expression of NF-E2 and its partner, MafG, in CD34-
derived normal (five cases) and malignant megakaryocytes
from essential thrombocythemia (ET) patients (eight cases) and
in megakaryoblastic cell lines. We also quantitated the mRNA
of the thromboxane synthase (TXS) gene, which is directly
regulated by NF-E2. Although real-time RT-PCR showed that
both a and f NF-E2 isoforms were significantly reduced with
respect to the normal counterpart both in ET megakaryocytes
and in cell lines (P < 0.01), western blotting revealed decreased
NF-E2 protein expression only in the latter. However, both the
NF-E2a/MafG mRNA ratio (P < 0.01) and TXS (P < 0.01) mRNA
expression were significantly reduced in megakaryocytes from
ET patients and cell lines with respect to healthy subjects.
These two findings provide strong indirect evidence of altered
activity of the a isoform of NF-E2 in malignant megakaryocytes,
raising the possibility that NF-E2 could play a role in
megakaryocyte transformation.
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