On the Relationship between Jetted Inks and Printed Biopatterns: Molecular-Thin Functional Microarrays of Glucose Oxidase (Articolo in rivista)

Type
Label
  • On the Relationship between Jetted Inks and Printed Biopatterns: Molecular-Thin Functional Microarrays of Glucose Oxidase (Articolo in rivista) (literal)
Anno
  • 2009-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1021/la900071z (literal)
Alternative label
  • Arrabito G; Musumeci C; Aiello V; Libertino S; Compagnini G; Pignataro B (2009)
    On the Relationship between Jetted Inks and Printed Biopatterns: Molecular-Thin Functional Microarrays of Glucose Oxidase
    in Langmuir
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Arrabito G; Musumeci C; Aiello V; Libertino S; Compagnini G; Pignataro B (literal)
Pagina inizio
  • 6312 (literal)
Pagina fine
  • 6318 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 25 (literal)
Rivista
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • 1. Univ Palermo, Dipartimento Chim Fis F Accascina, I-90128 Palermo, Italy 2. PLAST ICs, I-95121 Catania, Italy 3. Univ Catania, Dipartimento Sci Chim, I-95100 Catania, Italy 4. Superlab Consorzio Catania Ric, I-95121 Catania, Italy 5. Univ Catania, Dipartimento Chim Biol Chim Med & Biol Mol, I-95100 Catania, Italy 6. CNR IMM, I-95121 Catania, Italy (literal)
Titolo
  • On the Relationship between Jetted Inks and Printed Biopatterns: Molecular-Thin Functional Microarrays of Glucose Oxidase (literal)
Abstract
  • Arrays of circular spots of glucose oxidase have been obtained on functionalized silicon oxide by piezoelectric inkjet printing and the enzymatic activity toward glucose recognition has been monitored. The addition of glycerol to the molecular ink allows to obtain high spot definition and resolution (tens of micrometers wide; one molecule tall), but in spite of its well-known structural stabilizing properties, in dynamic conditions it may lead to increased protein stresses. The jetting voltage and pulse length have been found to be critical factors for both activity retention and pattern definition. High voltages and pulse lengths results in stress effects along with the loss of activity, which, at least in our experimental conditions, has been found to be recovered in time. (literal)
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