http://www.cnr.it/ontology/cnr/individuo/prodotto/ID36437

Electrical Characterization of Biological Molecules Deposition in MOS Capacitors (Articolo in rivista)

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Electrical Characterization of Biological Molecules Deposition in MOS Capacitors (Articolo in rivista) (literal)

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Libertino S. 1; Fichera M. 1; Aiello V. 1,2,3; Lombardo S. 1, Scandurra A. 4; Renis M. 2; Sinatra F. 3 (2008)
Electrical Characterization of Biological Molecules Deposition in MOS Capacitors
in Sensor letters (print)
(literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

Libertino S. 1; Fichera M. 1; Aiello V. 1,2,3; Lombardo S. 1, Scandurra A. 4; Renis M. 2; Sinatra F. 3 (literal)

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1 CNR-IMM sez. Catania, Stradale Primosole 50, 95121 Catania, Italy 2 Universita Degli Studi di Catania, Dipartimento di'Chimiea Biologiea, Chimiea Medica e Biologia Moleeolare, Viale A.Doria, 6, 95125 Catania, Italy 3 UniversitaDegli StudidiCatania,Dipartimento diSeienze Biomediehe, ViaS.Sofia, 87, 95100 Catania, Italy 4 Laboratorio SupetticleInterfasi(SUPERLAB), ConsorzioCataniaRieerehe, Stradale Primosole50, 95121Cataniq, Italy (literal)

Titolo

Electrical Characterization of Biological Molecules Deposition in MOS Capacitors (literal)

Abstract

The aim of this work was the electrical characterization of biological molecules covalently immobilized on the dielectric of a MOS-like structure. The experimental protocol to bond the biological molecules on SiO2 lies in: oxide activation, silanization, linker molecule deposition, biological molecule bonding. Both an enzyme, the glucose oxidase (GOx), and single-stranded oligonucleotides (ssDNA) were selected as biological molecules. The effectiveness of the immobilization procedure was tested by X-ray Photoelectron spectroscopy. The electrical characterization was carried out on reference and fully processed samples as a function of the electrolyte pH, from 3 to 8, and of measurement time, up to 200 s. The oxide does not experience any aging during the measurement sets for voltages up to +/- 3 V. GOx deposition produces a shift of about -0.7 +/- 0.04 V in the V-FB, suggesting the molecule has a positive charge when anchored to the oxide layer. On the other hand, ssDNA deposition causes a positive shift, below 0.3 V, as expected due to the DNA negative charge in solution. The hybridization process causes a further shift in the VFB above 0.4 V, well above the experimental errors, confirming the sensitivity of this device to monitor the hybridization. Our preliminary data show a potential for the development of MOS-based biosensors. (literal)

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