Immobilization of the enzyme glucose oxidase on both bulk and porous SiO2 surfaces (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Immobilization of the enzyme glucose oxidase on both bulk and porous SiO2 surfaces (Articolo in rivista) (literal)

Anno

• 2008-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.3390/s8095637 (literal)

Alternative label

• Libertino S. 1; Aiello V. 2,3; Scandurra A. 4; Renis M. 2, Sinatra F. 3 (2008)
  Immobilization of the enzyme glucose oxidase on both bulk and porous SiO2 surfaces
  in Sensors (Basel)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Libertino S. 1; Aiello V. 2,3; Scandurra A. 4; Renis M. 2, Sinatra F. 3 (literal)

Pagina inizio

• 5637 (literal)

Pagina fine

• 5648 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 8 (literal)

Rivista

• Sensors (Rivista)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• 1 CNR - IMM Stradale Primosole 50 Catania, Italy; 2 Università degli Studi di Catania, Dipartimento di Chimica Biologica, Chimica Medica e Biologia Molecolare, Catania, Italy; 3 Università degli Studi di Catania, Dipartimento di Scienze Biomediche, Catania, Italy; 4 Laboratorio Superfici e Interfasi (SUPERLAB), Consorzio Catania Ricerche, Catania, Italy; (literal)

Titolo

• Immobilization of the enzyme glucose oxidase on both bulk and porous SiO2 surfaces (literal)

Abstract

• Silicon dioxide surfaces, both bulk and porous, were used to anchor the enzyme glucose oxidase. The immobilization protocol was optimized and the samples characterized using X-ray Photoelectron Spectroscopy, Energy Dispersive X-rays coupled to scanning electron microscopy and enzymatic activity measurements. We show that a uniform layer was obtained by activating the oxide before immobilization. X-ray Photoelectron Spectroscopy measurements carried out on bulk oxide showed that the silicon substrate signal was fully screened after the enzyme deposition showing the absence of uncovered surface regions. The enzyme presence was detected monitoring both the C 1s and N 1s signals. Finally, enzymatic activity measurements confirmed that the glucose oxidase activity was preserved after immobilization and maintained after three months of shelf life if the sample was properly stored. The importance of using porous silicon oxide to maximize the surface area was also evidenced. (literal)

Prodotto di

• Institute for microelectronics and microsystems (IMM) (Istituto)
• Nuovi sensori in tecnologia planare (MD.P05.008.002) (Modulo)

Autore CNR

• SEBANIA LIBERTINO (Unità di personale interno)

Insieme di parole chiave

• Keywords of "Immobilization of the enzyme glucose oxidase on both bulk and porous SiO2 surfaces" (Insieme di parole chiave)

Incoming links:

Autore CNR di

• SEBANIA LIBERTINO (Unità di personale interno)

Prodotto

• Institute for microelectronics and microsystems (IMM) (Istituto)
• Nuovi sensori in tecnologia planare (MD.P05.008.002) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Sensors (Rivista)

Insieme di parole chiave di

• Keywords of "Immobilization of the enzyme glucose oxidase on both bulk and porous SiO2 surfaces" (Insieme di parole chiave)