XPS and AFM characterization of the enzyme glucose oxidase immobilized on SiO2 surfaces (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• XPS and AFM characterization of the enzyme glucose oxidase immobilized on SiO2 surfaces (Articolo in rivista) (literal)

Anno

• 2008-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1021/la7029664 (literal)

Alternative label

• Libertino S; Giannazzo F; Aiello V; Scandurra A; Sinatra F; Renis M; Fichera M (2008)
  XPS and AFM characterization of the enzyme glucose oxidase immobilized on SiO2 surfaces
  in Langmuir
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Libertino S; Giannazzo F; Aiello V; Scandurra A; Sinatra F; Renis M; Fichera M (literal)

Pagina inizio

• 1965 (literal)

Pagina fine

• 1972 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 24 (literal)

Rivista

• Langmuir (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 5 (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• [Libertino, Sebania; Giannazzo, Filippo; Aiello, Venera; Fichera, Manuela] CNR, IMM Unita Catania, I-95121 Catania, Italy; [Aiello, Venera; Renis, Marcella] Univ Catania, Dipartimento Chim Biol Chim Med & Biol Mol, I-95125 Catania, Italy; [Scandurra, Antonino] Consorzio Catania Ricerche, Lab Superfici & Interfasi SUPERLAB, I-95121 Catania, Italy; [Sinatra, Fulvia] Univ Catania, Dipartimento Sci Biomed, I-95100 Catania, Italy (literal)

Titolo

• XPS and AFM characterization of the enzyme glucose oxidase immobilized on SiO2 surfaces (literal)

Abstract

• A process to immobilize the enzyme glucose oxidase on SiO2 surfaces for the realization of integrated microbiosensors was developed. The sample characterization was performed by monitoring, step by step, oxide activation, silanization, linker molecule (glutaraldehyde) deposition, and enzyme immobilization by means of XPS, AFM, and contact angle measurements. The control of the environment during the procedure, to prevent silane polymerization, and the use of oxide activation to obtain a uniform enzyme layer are issues of crucial importance. The correct protocol application gives a uniform layer of the linker molecule and the maximum sample surface coverage. This result is fundamental for maximizing the enzyme bonding sites-on the sample surface and achieving the maximum surface coverage. Thin SiO2 layers thermally grown on a Si substrate were used. The XPS Si 2p signal of the. substrate was monitored during immobilization. Such a signal is not completely shielded by the thin oxide layer and it is fully suppressed after the completion of the whole protocol. A power spectral density analysis on the AFM measurements showed the crucial role of both the oxide activation and the intermediate steps (silanization and linker molecule deposition) to obtain uniform immobilized enzyme coverage. Finally, enzymatic activity measurements confirmed the suitability of the optimized protocol. (literal)

Prodotto di

• Institute for microelectronics and microsystems (IMM) (Istituto)
• Nuovi sensori in tecnologia planare (MD.P05.008.002) (Modulo)

Autore CNR

• FILIPPO GIANNAZZO (Persona)
• MANUELA FICHERA (Persona)
• SEBANIA LIBERTINO (Unità di personale interno)

Incoming links:

Autore CNR di

• SEBANIA LIBERTINO (Unità di personale interno)
• FILIPPO GIANNAZZO (Persona)
• MANUELA FICHERA (Persona)

Prodotto

• Institute for microelectronics and microsystems (IMM) (Istituto)
• Nuovi sensori in tecnologia planare (MD.P05.008.002) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Langmuir (Rivista)