Experimental characterization of proteins immobilized on Si-based materials (Articolo in rivista)

Type
Label
  • Experimental characterization of proteins immobilized on Si-based materials (Articolo in rivista) (literal)
Anno
  • 2007-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1016/j.mee.2006.10.064 (literal)
Alternative label
  • Libertino S; Fichera M; Aiello V; Statello G; Fiorenza P; Sinatra F (2007)
    Experimental characterization of proteins immobilized on Si-based materials
    in Microelectronic engineering
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Libertino S; Fichera M; Aiello V; Statello G; Fiorenza P; Sinatra F (literal)
Pagina inizio
  • 468 (literal)
Pagina fine
  • 473 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 84 (literal)
Rivista
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • CNR-IMM Catania, I-95121 Catania, Italy; Catania Univ, Dipartimento Sci Biomed, I-95100 Catania, Italy (literal)
Titolo
  • Experimental characterization of proteins immobilized on Si-based materials (literal)
Abstract
  • We studied the possibility to use techniques that are traditionally employed in microelectronics to detect biological molecules immobilized on and into Si-based materials having, as final goal, the structural characterization of a glucose biosensor. The inorganic immobilization surfaces used were both bulk and porous silicon dioxide and the biological molecule to monitor was the enzyme glucose oxidase, widely used as a sensing element in glucose biosensors. Bulk SiO2 was used to optimize the immobilization protocol and the step-by-step characterization was mainly carried out by Atomic Force Microscopy measurements. Once optimized, the same protocol was used to anchor the enzyme in a porous Si dioxide matrix. Traditional measurement techniques may fail in biological molecule detection since C, basic element of such biological molecules, is present in Si as a contaminant, or introduced during sample preparation, e.g. for TEM cross section analysis. The enzyme monitoring was carried out by electron diffraction X-ray measurements coupled with scanning electron microscopy and to be sure of the protein presence, it was previously labelled with gold nano particles. We believe that this la (literal)
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