Core-shell microspheres by dispersion polymerization as promising delivery systems for proteins (Articolo in rivista)

Type
Label
  • Core-shell microspheres by dispersion polymerization as promising delivery systems for proteins (Articolo in rivista) (literal)
Anno
  • 2005-01-01T00:00:00+01:00 (literal)
Alternative label
  • Sparnacci K., Laus M., Tondelli L., Bernardi C., Magnani L., Corticelli F., Marchisio M., Ensoli B., Castaldello A., Caputo A. (2005)
    Core-shell microspheres by dispersion polymerization as promising delivery systems for proteins
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Sparnacci K., Laus M., Tondelli L., Bernardi C., Magnani L., Corticelli F., Marchisio M., Ensoli B., Castaldello A., Caputo A. (literal)
Pagina inizio
  • 1557 (literal)
Pagina fine
  • 1574 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 16 (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Univ Piemonte Orinetale, Dpet Environm & Life Sci, INSTM, I-15100 Alessandria, Italy; CNR, ISOF, I-40129 Bologna, Italy; CNR, IMM, I-40129 Bologna, Italy; Univ Ferrara, Dept Morphol & Embriol, I-44100 Ferrara, Italy; Ist Super Sanita, I-00161 Rome, Italy; Univ Padua, Dept Histol Microbiol & Med Biotechnol, I-35122 Padua, Italy (literal)
Titolo
  • Core-shell microspheres by dispersion polymerization as promising delivery systems for proteins (literal)
Abstract
  • Functional poly(methyl methacrylate) core-shell microspheres were prepared by dispersion polymerization. An appropriate selection of experimental parameters and in particular of the initiator and stabilizer amount and of the medium solvency power allowed a monodisperse sample as large as 600 nm to be prepared. To this purpose, low initiator concentration, high steric stabilizer amount and a low solvency power medium were employed. The microspheres present a core-shell structure in which the outer shell is constituted by the steric stabilizer which affords carboxylic groups able to interact with basic proteins, such as trypsin, whose adsorption is essentially driven by the carboxylic group density in the microsphere shell. Finally, fluorescent microspheres were prepared for biodistribution studies and shown to be readily taken up by the cells both in vitro and in vivo. These results suggest that these microspheres are promising delivery systems for the development of novel protein-based vaccines. (literal)
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