Facilitated permeation of antibiotics across membrane channels - Interaction of the quinolone moxifloxacin with the OmpF channel (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Facilitated permeation of antibiotics across membrane channels - Interaction of the quinolone moxifloxacin with the OmpF channel (Articolo in rivista) (literal)

Anno

• 2008-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1021/ja803188c (literal)

Alternative label

• Mach, T; Neves, P; Spiga, E; Weingart, H; Winterhalter, M; Ruggerone, P; Ceccarelli, M; Gameiro, P (2008)
  Facilitated permeation of antibiotics across membrane channels - Interaction of the quinolone moxifloxacin with the OmpF channel
  in Journal of the American Chemical Society (Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Mach, T; Neves, P; Spiga, E; Weingart, H; Winterhalter, M; Ruggerone, P; Ceccarelli, M; Gameiro, P (literal)

Pagina inizio

• 13301 (literal)

Pagina fine

• 13309 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 130 (literal)

Rivista

• Journal of the American Chemical Society (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 9 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 40 (literal)

Note

• PubMe (literal)
• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• \"[Mach, Tivadar; Weingart, Helge; Winterhalter, Mathias] Jacobs Univ Bremen, D-28759 Bremen, Germany; [Spiga, Enrico; Ruggerone, Paolo; Ceccarelli, Matteo] Univ Cagliari, CNR, INFM SLACS, I-09042 Monserrato, CA, Italy; [Spiga, Enrico; Ruggerone, Paolo; Ceccarelli, Matteo] Univ Cagliari, Dipartimento Fis, I-09042 Monserrato, CA, Italy; [Neves, Patricia; Gameiro, Paula] Univ Porto, Fac Ciencias, P-4169007 Oporto, Portugal (literal)

Titolo

• Facilitated permeation of antibiotics across membrane channels - Interaction of the quinolone moxifloxacin with the OmpF channel (literal)

Abstract

• The facilitated influx of moxifloxacin through the most abundant channel in the outer cell wall of Gram-negative bacteria was investigated. Molecular modeling provided atomic details of the interaction with the channel surface, revealed the preferred orientation of the antibiotic along its pathway, and gave an estimated time necessary for translocation. High-resolution conductance measurements on single OmpF trimers allowed the passages of individual moxifloxacin molecules to be counted. The average mean residence time of 50 mu s is in agreement with the predicted strong interaction from the modeling. In contrast, control measurements with nalidixic acid, a hydrophobic antibiotic that rather permeates across the lipid membrane, revealed a negligible interaction. The spectral overlap of tryptophan with moxifloxacin was suitable for a FRET study of the protein-antibiotic interaction. Combining molecular dynamics simulations with selective quenching identified an interaction of moxifloxacin with Trp61 inside the OmpF channel, whereas nalidixic acid showed preferential interaction with Trp214 on the channel exterior. An understanding of the detailed molecular interactions between the antibiotic and its preferred channel may be used to develop new antibiotics with improved uptake kinetics. (literal)

Prodotto di

• Teoria e modeling computazionale di materiali e processi per le nanoscienze (MD.P06.014.001) (Modulo)
• Modelizzazione molecolare di sistemi biologici (MD.P06.026.001) (Modulo)

Autore CNR

• MATTEO CECCARELLI (Persona)
• PAOLO RUGGERONE (Unità di personale esterno)

Insieme di parole chiave

• Parole chiave di "Facilitated permeation of antibiotics across membrane channels - Interaction of the quinolone moxifloxacin with the OmpF channel" (Insieme di parole chiave)

Incoming links:

Autore CNR di

• PAOLO RUGGERONE (Unità di personale esterno)
• MATTEO CECCARELLI (Persona)

Prodotto

• Teoria e modeling computazionale di materiali e processi per le nanoscienze (MD.P06.014.001) (Modulo)
• Modelizzazione molecolare di sistemi biologici (MD.P06.026.001) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Journal of the American Chemical Society (Rivista)

Insieme di parole chiave di

• Parole chiave di "Facilitated permeation of antibiotics across membrane channels - Interaction of the quinolone moxifloxacin with the OmpF channel" (Insieme di parole chiave)