Analysis of hesperetin enantiomers in human urine after ingestion of blood orange juice by using nano-liquid chromatography (Articolo in rivista)

Type
Label
  • Analysis of hesperetin enantiomers in human urine after ingestion of blood orange juice by using nano-liquid chromatography (Articolo in rivista) (literal)
Anno
  • 2010-01-01T00:00:00+01:00 (literal)
Alternative label
  • K. Si-Ahmed, F. Tazerouti, A. Y. Badjah-Hadj-Ahmed, Z. Aturki, G. D'’Orazio, A. Rocco, S. Fanali (2010)
    Analysis of hesperetin enantiomers in human urine after ingestion of blood orange juice by using nano-liquid chromatography
    in Journal of pharmaceutical and biomedical analysis (Print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • K. Si-Ahmed, F. Tazerouti, A. Y. Badjah-Hadj-Ahmed, Z. Aturki, G. D'’Orazio, A. Rocco, S. Fanali (literal)
Pagina inizio
  • 225 (literal)
Pagina fine
  • 229 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 51 (literal)
Rivista
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • 1 - Institute of Chemical Methodologies, Italian National Council of Research, Area della Ricerca di Roma, Via Salaria Km 29,300, 00015 Monterotondo Scalo, Rome, Italy 2 - Laboratoire d'Analyse Organique Fonctionnelle, Faculté de Chimie, USTHB, B.P. 32, El Alia, Bab Ezzouar 16111, Alger, Algeria (literal)
Titolo
  • Analysis of hesperetin enantiomers in human urine after ingestion of blood orange juice by using nano-liquid chromatography (literal)
Abstract
  • Hesperetin (HT) is a flavanone abundantly found in citrus fruits. It has been reported thatHTpossesses significant antioxidant, anticancer, anti-inflammatory and analgesic activities. This explains the necessity of developing new methods more powerful and sensitive for analyzing HT in biological fluids. Taking into account the chiral nature of HT, the study of the stereospecific kinetics of in vitro and in vivo metabolism and tissue distribution could be a useful tool for further understanding stereoselective biotransformations in human body. A simple nano-liquid chromatographic method for the determination of the enantiomeric composition of hesperetin in human urine was developed. Chiral separation was achieved using a 100 micronmeter I.D. capillary, packed with phenyl-carbamate-propyl-beta-cyclodextrin stationary phase, employing a mobile phase composed by a mixture of triethylammonium acetate buffer (1%, v/v, pH 4.5) and water/methanol (30:70, v/v) at room temperature. The detection was done by using oncolumn UV detector at 205 nm. Calibration curves were linear in the studied concentration range from 0.25 to 25 microg/mL (r2 > 0.999). Precision assay was <4.5% and was within 3% at the limit of quantification (0.5 microg/mL). The recovery of 7-ethoxycoumarin (IS), R- and S-hesperetin was greater than 82.48%, utilizing a liquid–liquid extraction procedure. The developed method was successfully applied to the determination of hesperetin enantiomers in urine samples obtained from a male volunteer, after the ingestion of 1 L of a commercial blood orange juice. (literal)
Prodotto di
Autore CNR
Insieme di parole chiave

Incoming links:


Autore CNR di
Prodotto
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi
Insieme di parole chiave di
data.CNR.it