http://www.cnr.it/ontology/cnr/individuo/prodotto/ID3343
Dynamics of DNA adsorption on and release from SDS-DDAB cat-anionic vesicles: a multitechnique study (Articolo in rivista)
- Type
- Label
- Dynamics of DNA adsorption on and release from SDS-DDAB cat-anionic vesicles: a multitechnique study (Articolo in rivista) (literal)
- Anno
- 2008-01-01T00:00:00+01:00 (literal)
- Alternative label
Bonincontro, A; Falivene, M; La Mesa, C; Risuleo, G; Pena, MR (2008)
Dynamics of DNA adsorption on and release from SDS-DDAB cat-anionic vesicles: a multitechnique study
in Langmuir
(literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Bonincontro, A; Falivene, M; La Mesa, C; Risuleo, G; Pena, MR (literal)
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- Rivista
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- ISI Web of Science (WOS) (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- \"[Bonincontro, Adalberto; Falivene, Maria; Risuleo, Gianfranco] Univ Rome, CNISM, Dipartimento Fis, I-00185 Rome, Italy; [Bonincontro, Adalberto; La Mesa, Camillo; Risuleo, Gianfranco] Univ Rome, SOFT INFM CNR, Res Ctr, Dipartimento Fis, I-00185 Rome, Italy; [La Mesa, Camillo] Univ Rome, Dipartimento Chim, I-00185 Rome, Italy; [Risuleo, Gianfranco] Univ Rome, Dipartimento Genet & Biol Mol, I-00185 Rome, Italy; [Pena, Miriam Ruiz] Inst Super Tecnol & Ciencias Aplicadas, Havana 6163, Cuba (literal)
- Titolo
- Dynamics of DNA adsorption on and release from SDS-DDAB cat-anionic vesicles: a multitechnique study (literal)
- Abstract
- DNA adsorption and release from cat-anionic vesicles made of sodium dodecylsulfate-dodecyldimethylammonium bromide (SDS-DDAB) in nonstoichiometric amounts was investigated by different electrochemical, spectroscopic, and biomolecular strategies. The characterization of the vesicular system was performed by dynamic light scattering, which allowed estimating both its size and distribution function(s). The interaction dynamics was followed by dielectric spectroscopy and zeta-potential, as well as by agarose gel electrophoresis, AGE. Also, circular dichroism, CD, measurements were carried out, to ascertain possible structural rearrangements of DNA, consequent to the interactions with the cat-anionic vesicles. CD demonstrates that vesicle-bound DNA retains its native conformation. The results obtained by the aforementioned techniques are consistent and indicate that binding saturation is obtained at a [DNA/vesicles] charge ratio close to 0.8, considering only the excess surface charges on the vesicles. This result is apparently in contradiction with a purely electrostatic approach and is tentatively ascribed to the distance between charges on the biopolymer and the vesicle surface, respectively. A possible interpretation is discussed. The nucleic acid can be completely retrieved from the vesicles upon addition of adequate amounts of SDS, which is the defective surfactant in the vesicular system. Precipitation of the poorly soluble SD-DDA salt results in an almost complete release of DNA. (literal)
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