CEC separation of insect oostatic peptides using a strong-cation-exchange stationary phase (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• CEC separation of insect oostatic peptides using a strong-cation-exchange stationary phase (Articolo in rivista) (literal)

Anno

• 2007-01-01T00:00:00+01:00 (literal)

Alternative label

• Anna Rocco, Zeineb Aturki, Giovanni D’Orazio, Salvatore Fanali, Veronica Solínová, Jan Hlavácek, Václav Kasicka (2007)
  CEC separation of insect oostatic peptides using a strong-cation-exchange stationary phase
  in Electrophoresis (Weinh., Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Anna Rocco, Zeineb Aturki, Giovanni D’Orazio, Salvatore Fanali, Veronica Solínová, Jan Hlavácek, Václav Kasicka (literal)

Pagina inizio

• 1689 (literal)

Pagina fine

• 1695 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 28 (literal)

Rivista

• Electrophoresis (Rivista)

Note

• ISI Web of Science (WOS) (literal)

Titolo

• CEC separation of insect oostatic peptides using a strong-cation-exchange stationary phase (literal)

Abstract

• The separation of several insect oostatic peptides (IOPs) was achieved by using CEC with a strong-cation-exchange (SCX) stationary phase in the fused-silica capillary column of 75 mm id. The effect of organic modifier, ionic strength, buffer pH, applied voltage, and temperature on peptides’ resolution was evaluated. Baseline separation of the studied IOPs was achieved using a mobile phase containing 100 mM pH 2.3 sodium phosphate buffer/water/CAN (10:20:70 v/v/v). In order to reduce the analysis time, experiments were performed in the short side mode where the stationary phase was packed for 7 cm only. The selection of the experimental parameters strongly influenced the retention time, resolution, and retention factor. An acidic pH was selected in order to positively charge the analyzed peptides, the pI’s of which are about 3 in water buffer solutions. A goods electivity and resolution was achieved at pH < 2.8; at higher pH the three parameters decreased due to reduced or even zero charge of peptides. The increase in the ionic strength of the buffer present in the mobile phase caused a decrease in retention factor for all the studied compounds due to the decreased interaction between analytes and stationary phase. Raising the CAN concentration in the mobile phase in the range 40-80% v/v caused an increase in both retention factor, retention time, and resolution due to the hydrophilic interactions of IOPs with free silanols and sulfonic groups of the stationary phase. (literal)

Prodotto di

• Metodologie chimiche e radiochimiche per lo sviluppo di materiali e processi sostenibili (PM.P03.016.001) (Modulo)
• Istituto di metodologie chimiche (IMC) (Istituto)

Autore CNR

• SALVATORE FANALI (Unità di personale interno)
• ZEINEB ATURKI (Unità di personale interno)
• GIOVANNI D'ORAZIO (Persona)
• ANNA ROCCO (Persona)

Incoming links:

Autore CNR di

• ZEINEB ATURKI (Unità di personale interno)
• SALVATORE FANALI (Unità di personale interno)
• GIOVANNI D'ORAZIO (Persona)
• ANNA ROCCO (Persona)

Prodotto

• Istituto di metodologie chimiche (IMC) (Istituto)
• Metodologie chimiche e radiochimiche per lo sviluppo di materiali e processi sostenibili (PM.P03.016.001) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Electrophoresis (Rivista)