Use of teicoplanin stationary phase for the enantiomeric resolution of atenolol in human urine by nano-liquid chromatography–mass spectrometry. (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Use of teicoplanin stationary phase for the enantiomeric resolution of atenolol in human urine by nano-liquid chromatography–mass spectrometry. (Articolo in rivista) (literal)

Anno

• 2006-01-01T00:00:00+01:00 (literal)

Alternative label

• D’Orazio G., Fanali S. (2006)
  Use of teicoplanin stationary phase for the enantiomeric resolution of atenolol in human urine by nano-liquid chromatography–mass spectrometry.
  in Journal of pharmaceutical and biomedical analysis (Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• D’Orazio G., Fanali S. (literal)

Pagina inizio

• 539 (literal)

Pagina fine

• 544 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 40 (literal)

Rivista

• Journal of pharmaceutical and biomedical analysis (Rivista)

Note

• ISI Web of Science (WOS) (literal)

Titolo

• Use of teicoplanin stationary phase for the enantiomeric resolution of atenolol in human urine by nano-liquid chromatography–mass spectrometry. (literal)

Abstract

• Nano-liquid chromatography (nano-LC) was used for the enantiomeric resolution of atenolol employing a teicoplanin modified silica stationary phase prepared in our laboratory. Experiments were carried out in a fused silica capillary of 75 um i.d. packed with chiral modified silica particles of 5 um diameter. Separated enantiomers were revealed by on-line UV detector at 205 nm or electrospray-ion-trap mass spectrometer (ESI-MS). Atenolol enantiomers were eluted utilizing a mobile phase with the following composition: 500mM ammonium acetate pH 4.5/methanol/acetonitrile 1/60/39 (v/v/v) allowing to achieve good enantioresolution in a reasonable analysis time (about 8 min) with a flow rate of about 900 nL/min. After comparing the sensitivity of the nano-LC method using a conventional UV detector for capillary electrophoresis, a zeta cell (3 nL volume) employed in nano-LC and the ion-trap MS the method was validated with the MS detector offering the highest sensitivity (limit of detection (LOD) 50 ng/mL; limit of quantification (LOQ) 400 ng/mL for each atenolol enantiomer). Nor-ephedrine was used as the internal standard. The method was successfully applied to the analysis of atenolol enantiomers present in human urine samples of a patient under atenolol therapy. (literal)

Prodotto di

• Istituto di metodologie chimiche (IMC) (Istituto)
• Metodologie chimiche e radiochimiche per lo sviluppo di materiali e processi sostenibili (PM.P03.016.001) (Modulo)

Autore CNR

• SALVATORE FANALI (Unità di personale interno)
• GIOVANNI D'ORAZIO (Persona)

Insieme di parole chiave

• Keywords of "Use of teicoplanin stationary phase for the enantiomeric resolution of atenolol in human urine by nano-liquid chromatography–mass spectrometry." (Insieme di parole chiave)

Incoming links:

Prodotto

• Istituto di metodologie chimiche (IMC) (Istituto)
• Metodologie chimiche e radiochimiche per lo sviluppo di materiali e processi sostenibili (PM.P03.016.001) (Modulo)

Autore CNR di

• SALVATORE FANALI (Unità di personale interno)
• GIOVANNI D'ORAZIO (Persona)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Journal of pharmaceutical and biomedical analysis (Rivista)

Insieme di parole chiave di

• Keywords of "Use of teicoplanin stationary phase for the enantiomeric resolution of atenolol in human urine by nano-liquid chromatography–mass spectrometry." (Insieme di parole chiave)