Trascriptomic Profiling of the Development of the Inflammatory Response in Human Monocytes in vitro (Articolo in rivista)

Type
Label
  • Trascriptomic Profiling of the Development of the Inflammatory Response in Human Monocytes in vitro (Articolo in rivista) (literal)
Anno
  • 2014-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1371/journal.pone.0087680 (literal)
Alternative label
  • Italiani P1, Mazza EM2, Lucchesi D1, Cifola I1, Gemelli C2, Grande A2, Battaglia C3, Bicciato S2, Boraschi D4. (2014)
    Trascriptomic Profiling of the Development of the Inflammatory Response in Human Monocytes in vitro
    in PloS one
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Italiani P1, Mazza EM2, Lucchesi D1, Cifola I1, Gemelli C2, Grande A2, Battaglia C3, Bicciato S2, Boraschi D4. (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 9 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 2 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • 1Institute of Biomedical Technologies, National Research Council, Pisa/Segrate, Italy. 2Department of Life Sciences, University of Modena and Reggio Emilia, Modena, Italy. 3Institute of Biomedical Technologies, National Research Council, Pisa/Segrate, Italy ; Department of Medical Biotechnology and Translational Medicine, University of Milano, Segrate, Italy. 4Institute of Biomedical Technologies, National Research Council, Pisa/Segrate, Italy ; Institute of Protein Biochemistry, National Research Council, Naples, Italy. (literal)
Titolo
  • Trascriptomic Profiling of the Development of the Inflammatory Response in Human Monocytes in vitro (literal)
Abstract
  • Monocytes/macrophages are key players in all phases of physiological and pathological inflammation. To understanding the regulation of macrophage functional differentiation during inflammation, we designed an in vitro model that recapitulates the different phases of the reaction (recruitment, initiation, development, and resolution), based on human primary blood monocytes exposed to sequential changes in microenvironmental conditions. All reaction phases were profiled by transcriptomic microarray analysis. Distinct clusters of genes were identified that are differentially regulated through the different phases of inflammation. The gene sets defined by GSEA analysis revealed that the inflammatory phase was enriched in inflammatory pathways, while the resolution phase comprised pathways related to metabolism and gene rearrangement. By comparing gene clusters differentially expressed in monocytes vs. M1 and vs. M2 macrophages extracted from an in-house created meta-database, it was shown that cells in the model resemble M1 during the inflammatory phase and M2 during resolution. The validation of inflammatory and transcriptional factors by qPCR and ELISA confirmed the transcriptomic profiles in the different phases of inflammation. The accurate description of the development of the human inflammatory reaction provided by this in vitro kinetic model can help in identifying regulatory mechanisms in physiological conditions and during pathological derangements. (literal)
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