Investigation of immunoglobulins in skin of the Antartic teleost Trematomus bernacchii (Articolo in rivista)

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  • Investigation of immunoglobulins in skin of the Antartic teleost Trematomus bernacchii (Articolo in rivista) (literal)
Anno
  • 2014-01-01T00:00:00+01:00 (literal)
Alternative label
  • Coscia MR1, Simoniello P2, Giacomelli S3, Oreste U3, Motta CM4. (2014)
    Investigation of immunoglobulins in skin of the Antartic teleost Trematomus bernacchii
    in Fish & shellfish immunology (Print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Coscia MR1, Simoniello P2, Giacomelli S3, Oreste U3, Motta CM4. (literal)
Pagina inizio
  • 206 (literal)
Pagina fine
  • 214 (literal)
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  • 39 (literal)
Rivista
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  • 8 (literal)
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  • 1Institute of Protein Biochemistry, CNR, via P. Castellino 111, 80131 Naples, Italy. Electronic address: mr.coscia@ibp.cnr.it. 2Department of Biological Science, University of Naples Federico II, via Mezzocannone 8, 80134 Naples, Italy; GSI Helmholtzzentrum für Schwerionenforschung, Planckstrasse 1, Darmstadt, Germany. 3Institute of Protein Biochemistry, CNR, via P. Castellino 111, 80131 Naples, Italy. 4Department of Biological Science, University of Naples Federico II, via Mezzocannone 8, 80134 Naples, Italy. (literal)
Titolo
  • Investigation of immunoglobulins in skin of the Antartic teleost Trematomus bernacchii (literal)
Abstract
  • The presence and production of IgM in the skin of the Antarctic teleost Trematomus bernacchii were investigated in this study. Immunoglobulins purified from cutaneous mucus and analysed by SDS-PAGE run under non-reducing and reducing conditions, were composed of heavy and light chains of 78 kDa and 25 kDa respectively, with a relative molecular mass of 830 kDa indicating that mucus IgM are tetramers as the serum IgM. Mature transcripts encoding the constant domains of both the secretory and membrane-bound Ig? chain were seen in T. bernacchii skin using a PCR strategy and the expression of the secretory Ig? chain in the skin was compared with that in other tissues by Real-time PCR. Cytological investigations revealed the presence of either immunoglobulins or their transcripts in occasional lymphocytes distributed close to the basal membrane. IgM once produced here, enters the filament-containing cells and is released into the mucus when these cells degenerate and detach from the epidermis. Our findings indicate that a cutaneous defence mechanism, functioning as anatomical and physiological barrier under subzero conditions, is present in this Antarctic species as an important component of the immune system. (literal)
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