active accumulation of very diluted biomolecoles by nano-dispensing for easy detection below the femtomolar range (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• active accumulation of very diluted biomolecoles by nano-dispensing for easy detection below the femtomolar range (Articolo in rivista) (literal)

Anno

• 2014-01-01T00:00:00+01:00 (literal)

Alternative label

• 1)S. Grilli, 1) L. Miccio, 1) O. Gennari, 1) S. Coppola, 1)V. Vespini, 1)L. Battista, 2) P. Orlando & 3)P. Ferraro (2014)
  active accumulation of very diluted biomolecoles by nano-dispensing for easy detection below the femtomolar range
  in Nature communications; Ferraro, Pozzuoli (Italia)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• 1)S. Grilli, 1) L. Miccio, 1) O. Gennari, 1) S. Coppola, 1)V. Vespini, 1)L. Battista, 2) P. Orlando & 3)P. Ferraro (literal)

Pagina inizio

• 5314 (literal)

Pagina fine

• 5314 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 5 (literal)

Rivista

• Nature communications (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 1 (literal)

Note

• PubMed (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• 1National Institute of Optics, National Council of Research (CNR-INO), Via Campi Flegrei 34, 80078 Pozzuoli, Italy. 21] National Institute of Optics, National Council of Research (CNR-INO), Via Campi Flegrei 34, 80078 Pozzuoli, Italy [2] Institute of Protein Biochemistry, National Council of Research (CNR-IBP), Via Campi Flegrei 34, 80078 Pozzuoli, Italy. 31] National Institute of Optics, National Council of Research (CNR-INO), Via Campi Flegrei 34, 80078 Pozzuoli, Italy [2] CNR-INO &CNR, \"E. Caianiello\", Via Campi Flegrei 34, 80078 Pozzuoli (NA), Italy. (literal)

Titolo

• active accumulation of very diluted biomolecoles by nano-dispensing for easy detection below the femtomolar range (literal)

Abstract

• Highly sensitive detection of biomolecules is of paramount interest in many fields including biomedicine, safety and eco-pollution. Conventional analyses use well-established techniques with detection limits ~1 pM. Here we propose a pyro-concentrator able to accumulate biomolecules directly onto a conventional binding surface. The operation principle is relatively simple but very effective. Tiny droplets are drawn pyro-electro-dynamically and released onto a specific site, thus increasing the sensitivity. The reliability of the technique is demonstrated in case of labelled oligonucleotides diluted serially. The results show the possibility to detect very diluted oligonucleotides, down to a few hundreds of attomoles. Excellent results are shown also in case of a sample of clinical interest, the gliadin, where a 60-fold improved detection limit is reached, compared with standard ELISA. This method could open the way to a mass-based technology for sensing molecules at very low concentrations, in environmental as well as in diagnostics applications. (literal)

Editore

• Ferraro (Editore)

Prodotto di

• DR. D'AURIA: Progettazione e Sviluppo di Biochip per la Sicurezza Alimentare e Salute Umana (AG.P05.001.001) (Modulo)
• Istituto di biochimica delle proteine (IBP) (Istituto)

Incoming links:

Prodotto

• Istituto di biochimica delle proteine (IBP) (Istituto)
• DR. D'AURIA: Progettazione e Sviluppo di Biochip per la Sicurezza Alimentare e Salute Umana (AG.P05.001.001) (Modulo)

Editore di

• Ferraro (Editore)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Nature communications (Rivista)