A multidisciplinary approach to study proteins-humic substances interactions (Abstract/Poster in convegno)

Type
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  • A multidisciplinary approach to study proteins-humic substances interactions (Abstract/Poster in convegno) (literal)
Anno
  • 2011-01-01T00:00:00+01:00 (literal)
Alternative label
  • Mariarita Arenella; Grazia Masciandaro; Ilaria Palchetti; Sara Tombelli; Marco Mascini; Loretta Landi; Brunello Ceccanti; Paolo Nannipieri; Giancarlo Renella (2011)
    A multidisciplinary approach to study proteins-humic substances interactions
    in Ecology of Soil Microorganisms, Praga, Repubblica Ceca, 27 aprile -1 maggio 2011
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Mariarita Arenella; Grazia Masciandaro; Ilaria Palchetti; Sara Tombelli; Marco Mascini; Loretta Landi; Brunello Ceccanti; Paolo Nannipieri; Giancarlo Renella (literal)
Note
  • Poster (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Grazia Masciandaro, Brunello Ceccanti: CNR-ISE, Sede distaccata di Pisa; Mariarita Arenella, Loretta Landi, Paolo Nannipieri, Giancarlo Renella: Dipartimento di Scienza del Suolo e Nutrizione della Pianta, Università degli Studi di Firenze, Piazzale delle Cascine 28, 50144 Firenze, Italy; Ilaria Palchetti, Sara Tombelli, Marco Mascini: Department of Chemistry, University of Florence, Via della Lastruccia 3, 50019 Sesto Fiorentino (Fi) Italy (literal)
Titolo
  • A multidisciplinary approach to study proteins-humic substances interactions (literal)
Abstract
  • Humic substances is a generic term to indicate relatively high molecular weight, brown to black colored substances formed by secondary condensation reactions, negatively charged, branched polyelectrolytes with mainly carboxylic and phenolic functional groups. Humic substances are ubiquitary in nature. The classical view of humic substances shares three basic fractions termed humic acids (soluble at alkaline pH values), fulvic acids (soluble at a wide range of pH values) and humine (insoluble in water at any pH). Considerable uncertainty still exists regarding the molecular weight and structure of humic substance. The main accepted model is the supramolecular structure, and regardless of their molecular structure, they constitute 70-80% of the soil organic matter. It is well kwon that proteins in the soil ecosystem interact with the solid phases, either inorganic (e.g. clays) or organic (e.g. humic acids). While interactions between proteins of various origin with clays has been widely studied, interactions between proteins and humic substances is considerably less characterized. Most of the information on this topic is based on the extraction of humic-enzymatic complexes, which have been proved to preserve the enzymes from chemical degradation or hydrolysis. To understand the modes of interactions between microbial proteins and humic substances, we undertook a multidisciplinary study based on the behaviour of humic substances of various origin, with a purified ?-glucosidase from the soil-borne fungus Aspergillus niger. Chemical interactions between the enzyme and humic substances was studied using a quartz microbalance (Kinetica, Elba Tech), which indicates the eventual formation of chemical bonds. Effects of the humic substances on the enzyme activity was studied through native gel electrophoresis and visualization of the enzyme activity by staining with 4-Methylumbelliferyl-beta-D-glucopyranoside. The preliminary results show that under the tested conditions, the formation of enzyme-humic substances complexes requires specific factors to occur, and that the ?-glucosidase activity is apparently not influenced by the incubation with HS, confirming the lack of something enhancing the reaction between the partners. (literal)
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