Culture and Characterization of Mammary Cancer Stem Cells in Mammospheres (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Culture and Characterization of Mammary Cancer Stem Cells in Mammospheres (Articolo in rivista) (literal)

Anno

• 2015-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1007/978-1-4939-1785-3_18 (literal)

Alternative label

• Piscitelli, Eleonora; Cocola, Cinzia; Thaden, Frank Ruediger; Pelucchi, Paride; Gray, Brian; Bertalot, Giovanni; Albertini, Alberto; Reinbold, Rolland; Zucchi, Ileana (2015)
  Culture and Characterization of Mammary Cancer Stem Cells in Mammospheres
  in Methods in molecular biology (Clifton N.J.)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Piscitelli, Eleonora; Cocola, Cinzia; Thaden, Frank Ruediger; Pelucchi, Paride; Gray, Brian; Bertalot, Giovanni; Albertini, Alberto; Reinbold, Rolland; Zucchi, Ileana (literal)

Pagina inizio

• 243 (literal)

Pagina fine

• 262 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 1235 (literal)

Rivista

• Methods in molecular biology (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 20 (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Segrate Milan; Mol Targeting Technol Inc; Azienda Osped Desenzano DG (literal)

Titolo

• Culture and Characterization of Mammary Cancer Stem Cells in Mammospheres (literal)

Abstract

• Mammospheres (MMs) are a model for culturing and maintaining mammary gland stem cells (SCs) or cancer stem cells (CSCs) ex situ. As MMs recapitulate the micro-niche of the mammary gland or a tumor, MMs are a model for studying the properties of SCs or CSCs, and for mapping, isolating, and characterizing the SC/CSC generated lineages. Cancer stem cells share with normal SCs the properties of self-renewal and the capacity to generate all cell types and organ structures of the mammary gland. Analysis of human tumor samples suggests that CSCs are heterogeneous in terms of proliferation and differentiation potential. Mammospheres from CSCs likewise display heterogeneity. This heterogeneity makes analysis of CSC generated MMs challenging. To identify the unique and diverse properties of MM derived CSCs, comparative analysis with MMs obtained from normal SCs is required. Here we present protocols for identifying and enriching cells with SC features from a cancer cell line using the LA7CSCs as a model. A comprehensive and comparative approach for identifying, isolating, and characterizing MMs from SCs and CSCs from human breast is also introduced. In addition, we describe detailed procedures for identifying, isolating, and characterizing mammary gland specific cell types, generated during MM formation. (literal)

Prodotto di

• Oncologia molecolare : differenziamento cellule epiteliali, caratterizzazione cellule staminali mammarie. (ME.P03.010.001) (Modulo)

Autore CNR

• ELEONORA PISCITELLI (Unità di personale esterno)
• ALBERTO ALBERTINI (Persona)
• ROLLAND ALVONS REINBOLD (Persona)
• CINZIA COCOLA (Unità di personale interno)
• PARIDE PELUCCHI (Persona)
• ILEANA ZUCCHI (Persona)

Insieme di parole chiave

• Keywords of "Culture and Characterization of Mammary Cancer Stem Cells in Mammospheres" (Insieme di parole chiave)

Incoming links:

Autore CNR di

• ILEANA ZUCCHI (Persona)
• ALBERTO ALBERTINI (Persona)
• PARIDE PELUCCHI (Persona)
• ROLLAND ALVONS REINBOLD (Persona)
• ELEONORA PISCITELLI (Unità di personale esterno)
• CINZIA COCOLA (Unità di personale interno)

Prodotto

• Oncologia molecolare : differenziamento cellule epiteliali, caratterizzazione cellule staminali mammarie. (ME.P03.010.001) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Methods in molecular biology (Rivista)

Insieme di parole chiave di

• Keywords of "Culture and Characterization of Mammary Cancer Stem Cells in Mammospheres" (Insieme di parole chiave)