Inhibition of protein kinase CK2 by quercetin enhances CD95-mediated apoptosis in a human thymus-derived T cell line (Articolo in rivista)

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Label
  • Inhibition of protein kinase CK2 by quercetin enhances CD95-mediated apoptosis in a human thymus-derived T cell line (Articolo in rivista) (literal)
Anno
  • 2014-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1016/j.foodres.2014.05.022 (literal)
Alternative label
  • Russo, Maria; Spagnuolo, Carmela; Bilotto, Stefania; Tedesco, Idolo; Maiani, Giuseppe; Russo, Gian Luigi (2014)
    Inhibition of protein kinase CK2 by quercetin enhances CD95-mediated apoptosis in a human thymus-derived T cell line
    in Food research international
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Russo, Maria; Spagnuolo, Carmela; Bilotto, Stefania; Tedesco, Idolo; Maiani, Giuseppe; Russo, Gian Luigi (literal)
Pagina inizio
  • 244 (literal)
Pagina fine
  • 251 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 63 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali
  • 8 (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Consiglio Nazionale delle Ricerche (CNR); Res Ctr Food & Nutr CRA NUT (literal)
Titolo
  • Inhibition of protein kinase CK2 by quercetin enhances CD95-mediated apoptosis in a human thymus-derived T cell line (literal)
Abstract
  • Quercetin (3,3',4',5,7-pentahydroxyflavone) is one of the major dietary flavonoid, found in a broad range of fruits, vegetables and beverages, such as tea and wine, with a daily uptake between 10 and 100 mg depending on the dietary attitude. We previously demonstrated that quercetin sensitizes HPB-ALL cell line to CD95-mediated apoptosis through a mechanism independent of its antioxidant properties and related to its ability to regulate the activity of serine-threonine protein kinases. Here, we report that quercetin is an inhibitor of protein kinase CK2, a kinase involved in apoptotic resistance in several forms of leukemia. Quercetin inhibits CK2 in an in vitro assay and in HPB-ALL cells decreasing its activity of 3.5-fold within 12 h from treatment, in good agreement with the intracellular concentration of the molecule measured by HPLC on the same cells (0.933 +/- 0.188 mu g/mg of total proteins). Quercetin also inhibits pure CK2 enzyme with an EC50 of about 150 nM in an in vitro assay. A specific CK2 inhibitor, TBB (4,5,6,7-tetra-bromo-benzo-triazole), behaves similarly to quercetin since it abolishes CK2 activity in HPB-ALL cells, but fails to enhance CD95-mediated apoptosis when associated with an anti-CD95 monoclonal antibody mimicking CD95 ligand. The resistance to apoptotic induction in HPB-ALL can be bypassed taking advantage of the pleiotropic activity of quercetin. Combined treatments which include quercetin in association with death receptor inducers may represent a novel therapeutic strategy against leukemia. (C) 2014 Elsevier Ltd. All rights reserved. (literal)
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