Optical Tweezers for Synchrotron Radiation Probing of Trapped Biological and Soft Matter Objects in Aqueous Environments (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Optical Tweezers for Synchrotron Radiation Probing of Trapped Biological and Soft Matter Objects in Aqueous Environments (Articolo in rivista) (literal)

Anno

• 2011-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1021/ac200515x (literal)

Alternative label

• Santucci, Silvia C.; Cojoc, Dan; Amenitsch, Heinz; Marmiroli, Benedetta; Sartori, Barbara; Burghammer, Manfred; Schoeder, Sebastian; DiCola, Emanuela; Reynolds, Michael; Riekel, Christian (2011)
  Optical Tweezers for Synchrotron Radiation Probing of Trapped Biological and Soft Matter Objects in Aqueous Environments
  in Analytical chemistry (Wash.)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Santucci, Silvia C.; Cojoc, Dan; Amenitsch, Heinz; Marmiroli, Benedetta; Sartori, Barbara; Burghammer, Manfred; Schoeder, Sebastian; DiCola, Emanuela; Reynolds, Michael; Riekel, Christian (literal)

Pagina inizio

• 4863 (literal)

Pagina fine

• 4870 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 83 (literal)

Rivista

• Analytical chemistry (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 8 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 12 (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• European Synchrotron Radiat Facil; Ist Officina Mat; Austrian Academy of Sciences (literal)

Titolo

• Optical Tweezers for Synchrotron Radiation Probing of Trapped Biological and Soft Matter Objects in Aqueous Environments (literal)

Abstract

• Investigations of single fragile objects manipulated by optical forces with high brilliance X-ray beams may initiate the development of new research fields such as protein crystallography in an aqueous environment. We have developed a dedicated optical tweezers setup with a compact, portable, and versatile geometry for the customary manipulation of objects for synchrotron radiation applications. Objects of a few micrometers up to a few tens of micrometers size can be trapped for extended periods of time. The selection and positioning of single objects out of a batch of many can be performed semi-automatically by software routines. The performance of the setup has been tested by wide-angle and small-angle X-ray scattering experiments on single optically trapped starch granules, using a synchrotron radiation microbeam. We demonstrate here for the first time the feasibility of microdiffraction on optically trapped protein crystals. Starch granules and insulin crystals were repeatedly raster-scanned at about 50 ms exposure/raster-point up to the complete loss of the structural order. Radiation damage in starch granules results in the appearance of low-angle scattering due to the breakdown of the polysaccharide matrix. For insulin crystals, order along the densely packed [110] direction is preferentially maintained until complete loss of long-range order. (literal)

Prodotto di

• MD.P06.017.003 Manipolazione ottica applicata alle nano-biotecnologie (MD.P06.017.007) (Modulo)

Autore CNR

• SILVIA SANTUCCI (Persona)
• DANUT ADRIAN COJOC (Unità di personale interno)

Incoming links:

Autore CNR di

• DANUT ADRIAN COJOC (Unità di personale interno)
• SILVIA SANTUCCI (Persona)

Prodotto

• MD.P06.017.003 Manipolazione ottica applicata alle nano-biotecnologie (MD.P06.017.007) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Analytical chemistry (Rivista)