ALK expression defines a distinct group of T/null lymphomas (\"ALK lymphomas\") with a wide morphological spectrum (Articolo in rivista)

Type
Label
  • ALK expression defines a distinct group of T/null lymphomas (\"ALK lymphomas\") with a wide morphological spectrum (Articolo in rivista) (literal)
Anno
  • 1998-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1016/S0002-9440(10)65629-5 (literal)
Alternative label
  • Falini, B; Bigerna, B; Fizzotti, M; Pulford, K; Pileri, SA; Delsol, G; Carbone, A; Paulli, M; Magrini, U; Menestrina, F; Giardini, R; Pilotti, S; Mezzelani, A; Ugolini, B; Billi, M; Pucciarini, A; Pacini, R; Pelicci, PG; Flenghi, L (1998)
    ALK expression defines a distinct group of T/null lymphomas ("ALK lymphomas") with a wide morphological spectrum
    in The American journal of pathology (Print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Falini, B; Bigerna, B; Fizzotti, M; Pulford, K; Pileri, SA; Delsol, G; Carbone, A; Paulli, M; Magrini, U; Menestrina, F; Giardini, R; Pilotti, S; Mezzelani, A; Ugolini, B; Billi, M; Pucciarini, A; Pacini, R; Pelicci, PG; Flenghi, L (literal)
Pagina inizio
  • 875 (literal)
Pagina fine
  • 886 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 153 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali
  • 12 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 3 (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • University of Perugia; University of Perugia; University of Oxford; University of Bologna; Purpan Hosp; Ctr Oncol Aviano; University of Pavia; University of Verona; Fondazione IRCCS Istituto Nazionale Tumori Milan; European Institute of Oncology (literal)
Titolo
  • ALK expression defines a distinct group of T/null lymphomas (\"ALK lymphomas\") with a wide morphological spectrum (literal)
Abstract
  • The t(2;5)(p23;q35) translocation associated with CD30-positive anaplastic large cell lymphoma results in the production of a NPM-ALK chimeric protein, consisting of the N-terminal portion of the NPM protein joined to the entire cytoplasmic domain of the neural receptor tyrosine kinase ALK. The ALK gene products were identified in paraffin sections by using a new anti-ALK (cytoplasmic portion) monoclonal antibody (ALKc) that tends to react more strongly than a previously described ALK1 antibody with the nuclei of ALK-expressing tumor cells after microwave heating in 1 mmol/L ethylenediaminetetraacetic add buffer, pH 8.0. The ALKc monoclonal antibody reacted selectively with 60% of anaplastic large cell lymphoma cases (60 of 100), which occurred mainly in the first three decades of life and consistently displayed a T/null phenotype. This group of ALK-positive tumors showed a wide morphological spectrum including cases with features of anaplastic large cell lymphoma \"common\" type (75%), \"lymphohistiocytic\" (10%), \"small cell\" (8.3%), \"giant cell\" (3.3%), and \"Hodgkin's like\" (3.3%). CD30-positive large anaplastic cells expressing the ALK protein both in the cytoplasm and nucleus represented the dominant tumor population in the common, Hodgkin's-like and percentage (often with a perivascular distribution) also in cases with lymphohistiocytic and small cell features. In this study, the ALKc antibody also allowed us to identify small neoplastic cells (usually CD30 negative) with nucleus-restricted ALK positivity that were, by definition, more evident in the small cell variant but were also found in cases with lymphohistiocytic, common, and \"Hodgkin's-like\" features. These findings, which have not been previously emphasized, strongly suggest that the neoplastic lesion (the NPM-ALK gene) must be present both in the large anaplastic and small tumor cells, and that ALK-positive lymphomas lie on a spectrum, their position being defined by the ratio of small to large neoplastic cells. Notably, about 15% of all ALK-positive lymphomas (usually of the common or giant cell variant) showed a cytoplasm-restricted ALK positivity, which suggests that the ALK gene may have fused with a partner(s) other than NPM. From a diagnostic point of view, detection of the ALK protein was useful in distinguishing anaplastic large cell lymphoma cases of lymphohistiocytic and small cell variants from reactive conditions and other peripheral T-cell lymphoma subtypes, as well as for detecting a small number of tumor cells in lymphohemopoietic tissues. In conclusion, ALK positivity appears to define a clinicopathological entity with a T/null phenotype (\"ALK lymphomas\"), but one that shows a wider spectrum of morphological patterns than has been appreciated in the past. (literal)
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