Detection of xanthine oxidase activity products by EPR and HPLC in bronchoalveolar lavage fluid from patients with chronic obstructive pulmonary disease (Articolo in rivista)

Type
Label
  • Detection of xanthine oxidase activity products by EPR and HPLC in bronchoalveolar lavage fluid from patients with chronic obstructive pulmonary disease (Articolo in rivista) (literal)
Anno
  • 1998-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1016/S0891-5849(98)00128-2 (literal)
Alternative label
  • S. Pinamonti, M. Leis, A. Barbieri, D. Leoni, M. Muzzoli, S. Sostero, M.C. Chicca, A. Carrieri, F. Ravenna, L.M. Fabbri, A. Ciaccia (1998)
    Detection of xanthine oxidase activity products by EPR and HPLC in bronchoalveolar lavage fluid from patients with chronic obstructive pulmonary disease
    in Free radical biology & medicine
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • S. Pinamonti, M. Leis, A. Barbieri, D. Leoni, M. Muzzoli, S. Sostero, M.C. Chicca, A. Carrieri, F. Ravenna, L.M. Fabbri, A. Ciaccia (literal)
Pagina inizio
  • 771 (literal)
Pagina fine
  • 779 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 25 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 7 (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Pinamonti, S (Reprint Author), Univ Ferrara, Dept Biol, Evolutionary Biol Branch, Via L Borsari 46, I-44100 Ferrara, Italy. Univ Ferrara, Dept Biol, Evolutionary Biol Branch, I-44100 Ferrara, Italy. Univ Ferrara, Photoreact & Catalysis Ctr, Natl Res Council, I-44100 Ferrara, Italy. Univ Ferrara, Dept Chem, I-44100 Ferrara, Italy. Univ Ferrara, Dept Resp Dis, I-44100 Ferrara, Italy. (literal)
Titolo
  • Detection of xanthine oxidase activity products by EPR and HPLC in bronchoalveolar lavage fluid from patients with chronic obstructive pulmonary disease (literal)
Abstract
  • Xanthine oxidase (xanthine: oxygen oxidoreductase, EC 1.1.3.22), a molybdenum-containing hydroxylase that produces superoxide and uric acid from purine substrates and molecular oxygen, is involved in the oxidative stress underlying several human pathologies including lung diseases. An enzymatic activity similar to xanthine oxidase was previously reported in bronchoalveolar lavage fluid of patients with chronic obstructive pulmonary disease (COPD-BAL), by fluorometric analysis of DNA unwinding and cytochrome c reduction kinetics. Here we report the detection of xanthine oxidase activity products by electron paramagnetic resonance (EPR) in presence of the spin-trap 5,5-dimethyl-1-pyrroline N-oxide (DMPO) and reversed-phase high-performance liquid chromatography (RP-HPLC) in COPD-BAL (n = 14, average age of patients 65 years, range 38-81) and BAI, from healthy nonsmoker controls (n = 6, average age 64 years, range 44-73). Superoxide DMPO adducts were detected in COPD-BAL and in an in vitro system containing xanthine and xanthine oxidase (XA/XO), but not in BAL controls and when superoxide dismutase (SOD, 1000 I.U./ml) was added to COPD-BAL. The HPLC analyses after addition of xanthine showed production of uric acid in COPD-BAL and in the XA/XO system but not in BAL controls. These results support the involvement of xanthine oxidase in the mechanisms of superoxide production by BAL supernatant, which increases oxidative stress in chronic obstructive pulmonary disease. (C) 1998 Elsevier Science Inc. (literal)
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