http://www.cnr.it/ontology/cnr/individuo/prodotto/ID31953
The Role of Hydroxyapatite as Solid Signal on Performance of PCL Porous Scaffolds for Bone Tissue Regeneration (Articolo in rivista)
- Type
- Label
- The Role of Hydroxyapatite as Solid Signal on Performance of PCL Porous Scaffolds for Bone Tissue Regeneration (Articolo in rivista) (literal)
- Anno
- 2008-01-01T00:00:00+01:00 (literal)
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- Guarino V.; Causa F.; Netti P.A.; Ciapetti G.; Pagani S.; Martini D.; Baldini N.; Ambrosio L.; (literal)
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- Rivista
- Note
- ISI Web of Science (WOS) (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- Institute of Composite and Biomedical Materials, National Research Council and CRIB, University of Naples Federico II
Piazzale Tecchio 80, 80125 Naples, Italy
Department of Experimental and Clinical Medicine, University of Magna Graecia, Germaneto, 88100 Catanzaro, Italy
Laboratory for Pathophysiology of Orthopaedic Implants, Istituti Ortopedici Rizzoli, via di Barbiano 1/10,
40136 Bologna, Italy
Department of Human Anatomy, University of Bologna, via Irnerio 48, 40126 Bologna, Italy (literal)
- Titolo
- The Role of Hydroxyapatite as Solid Signal on Performance of PCL Porous Scaffolds for Bone Tissue Regeneration (literal)
- Abstract
- Highly porous composites made up of biodegradable poly-e-caprolactone (PCL) and stoichiometric hydroxyapatite (HA) particles have been developed as substrate for bonetissue regeneration. The processing technique consists of phase inversion and particulate (salt crystals) leaching. Three different HA contents (13, 20 and 26 vol %) in PCL-based composite were considered in this study. Pore microstructure with fully interconnected network and pore sizes ranging around a few hundred of lm (macroporosity) was obtained as a result of salt particles removal by leaching process. Several microns (microporosity) porosity was also
created through phase inversion of polymer solution. Total porosity up to 95% was achieved. Human marrow stromal cells (MSC) were seeded onto porous PCL-based composites for 15 weeks and cultured in osteogenic medium. MSC were able to adhere and grow on PCL-based substrates with a plateau at 34 weeks. However, the small effect of bioactive signals on the biological response evaluated in MSC cell culture suggests a prior role of topography on the biological response. Importantly, the presence of HA as a bioactive solid signal determines an increase of mechanical properties. On the overall, the results indicated that porous PCL-based
composites are potential candidate for bone substitution with beneficial influence on structural characteristics by solid signal addition. (literal)
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