http://www.cnr.it/ontology/cnr/individuo/prodotto/ID318899
Correction: High throughput approaches reveal splicing of primary microRNA transcripts and tissue specific expression of mature microRNAs in Vitis vinifera (Articolo in rivista)
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- Correction: High throughput approaches reveal splicing of primary microRNA transcripts and tissue specific expression of mature microRNAs in Vitis vinifera (Articolo in rivista) (literal)
- Anno
- 2010-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1186/1471-2164-11-109 (literal)
- Alternative label
Mica, Erica; Piccolo, Viviana; Delledonne, Massimo; Ferrarini, Alberto; Pezzotti, Mario; Casati, Cesare; Del Fabbro, Cristian; Valle, Giorgio Delia; Policriti, Alberto; Morgante, Michele; Pesole, Graziano; Pè, Mario Enrico Nrico; Horner, David Stephen (2010)
Correction: High throughput approaches reveal splicing of primary microRNA transcripts and tissue specific expression of mature microRNAs in Vitis vinifera
in BMC genomics
(literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Mica, Erica; Piccolo, Viviana; Delledonne, Massimo; Ferrarini, Alberto; Pezzotti, Mario; Casati, Cesare; Del Fabbro, Cristian; Valle, Giorgio Delia; Policriti, Alberto; Morgante, Michele; Pesole, Graziano; Pè, Mario Enrico Nrico; Horner, David Stephen (literal)
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- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
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- Universita degli Studi di Milano; Scuola Superiore Sant'Anna di Studi Universitari e di Perfezionamento; Universita degli Studi di Verona; Universita degli Studi di Udine; Universita degli Studi di Padova; Istituto di Genomica Applicata; Consiglio Nazionale delle Ricerche; Universita degli Studi di Bari (literal)
- Titolo
- Correction: High throughput approaches reveal splicing of primary microRNA transcripts and tissue specific expression of mature microRNAs in Vitis vinifera (literal)
- Abstract
- Background: MicroRNAs are short (~21 base) single stranded RNAs that, in plants, are generally coded by specific genes and cleaved specifically from hairpin precursors. MicroRNAs are critical for the regulation of multiple developmental, stress related and other physiological processes in plants. The recent annotation of the genome of the grapevine (Vitis vinifera L.) allowed the identification of many putative conserved microRNA precursors, grouped into multiple gene families.Results: Here we use oligonucleotide arrays to provide the first indication that many of these microRNAs show differential expression patterns between tissues and during the maturation of fruit in the grapevine. Furthermore we demonstrate that whole transcriptome sequencing and deep-sequencing of small RNA fractions can be used both to identify which microRNA precursors are expressed in different tissues and to estimate genomic coordinates and patterns of splicing and alternative splicing for many primary miRNA transcripts.Conclusions: Our results show that many microRNAs are differentially expressed in different tissues and during fruit maturation in the grapevine. Furthermore, the demonstration that whole transcriptome sequencing can be used to identify candidate splicing events and approximate primary microRNA transcript coordinates represents a significant step towards the large-scale elucidation of mechanisms regulating the expression of microRNAs at the transcriptional and post-transcriptional levels. © 2010 Mica et al; licensee BioMed Central Ltd. (literal)
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