Stress response gene activation elicited by X-rays in sea urchin embryos (Abstract/Poster in atti di convegno)

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  • Stress response gene activation elicited by X-rays in sea urchin embryos (Abstract/Poster in atti di convegno) (literal)
Anno
  • 2011-01-01T00:00:00+01:00 (literal)
Alternative label
  • R. Bonaventura1, F. Zito1, C. Costa1, S. Giarrusso2, F. Celi2, V. Matranga1 (2011)
    Stress response gene activation elicited by X-rays in sea urchin embryos
    in Italian Society of Space Biomedicine and Biotecnology, V Congresso Nazionale, Spazio La Nuova Frontiera per l'Umanità, Padova, 17-19 Novembre
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • R. Bonaventura1, F. Zito1, C. Costa1, S. Giarrusso2, F. Celi2, V. Matranga1 (literal)
Note
  • Poster (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • 1 Istituto di Biomedicina e Immunologia Molecolare \"A. Monroy\", Consiglio Nazionale delle Ricerche, Palermo, Italy 2 Istituto Nazionale di AstroFisica, Istituto di Astro?sica Spaziale e Fisica Cosmica, Palermo, Italy (literal)
Titolo
  • Stress response gene activation elicited by X-rays in sea urchin embryos (literal)
Abstract
  • We used Paracentrotus lividus sea urchin embryos, a well-established model system for classical and molecular eco-toxicological studies, to evaluate the effect of harmful ionizing radiation, such as X-rays. Cleavage embryos were exposed to X-rays at doses ranging from 0.1 to 5 Gy. We found a dose-dependent increase in developmental delays and severe morphological defects in embryos observed at 24 and 48h after irradiation. In parallel, we measured a dose-dependent reduction in the mRNA levels of two skeleton-speci?c genes, Pl-SM30 and Pl-msp130. We also analyzed the accumulation levels of stress/anti-apoptotic proteins such as HSP60, HSP70, BAG3 and p63 when embryos were exposed to the high dose (5Gy) and harvested at 24 and 48h after irradiation. We found an increase in the HSP70, BAG3, and p63 protein levels only 48 h after irradiation, whereas no HSP60 increase was detected either at 24 or 48 h. Levels of the mRNA coding for HSP70 and p63 were also investigated by relative RT-PCR and were found to increase 24h after irradiation, returning to their initial levels at 48h. Results demonstrate the presence of an adaptive regulatory mechanism operating at the transcriptional level at 24h, followed by a translational activation at 48h post-irradiation. In conclusion, we propose the sea urchin embryo as a valid bio-indicator for monitoring the effects of radiations and as a test organism for studying the protective pathways operating after irradiation. This work has been fully supported by the MoMA Project (ASI, Contract 1/014/06/0) and partially by the Biomintec Project (EU 7thFP, Contract N° PITN-GA-2008-215507). (literal)
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