Single-Molecule Folding Mechanism of an EF-Hand Neuronal Calcium Sensor (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Single-Molecule Folding Mechanism of an EF-Hand Neuronal Calcium Sensor (Articolo in rivista) (literal)

Anno

• 2013-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1016/j.str.2013.07.022 (literal)

Alternative label

• Heidarsson, Petur O.; Otazo, Mariela R.; Bellucci, Luca; Mossa, Alessandro; Imparato, Alberto; Paci, Emanuele; Corni, Stefano; Di Felice, Rosa; Kragelund, Birthe B.; Cecconi, Ciro (2013)
  Single-Molecule Folding Mechanism of an EF-Hand Neuronal Calcium Sensor
  in Structure (Lond.)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Heidarsson, Petur O.; Otazo, Mariela R.; Bellucci, Luca; Mossa, Alessandro; Imparato, Alberto; Paci, Emanuele; Corni, Stefano; Di Felice, Rosa; Kragelund, Birthe B.; Cecconi, Ciro (literal)

Pagina inizio

• 1812 (literal)

Pagina fine

• 1821 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 21 (literal)

Rivista

• Structure (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 10 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 10 (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• University of Copenhagen; Consiglio Nazionale delle Ricerche (CNR)NANO; Ctr Appl Technol & Nucl Dev CEADEN; Aarhus University; University of Leeds (literal)

Titolo

• Single-Molecule Folding Mechanism of an EF-Hand Neuronal Calcium Sensor (literal)

Abstract

• EF-hand calcium sensors respond structurally to changes in intracellular Ca2+ concentration, triggering diverse cellular responses and resulting in broad interactomes. Despite impressive advances in decoding their structure-function relationships, the folding mechanism of neuronal calcium sensors is still elusive. We used single-molecule optical tweezers to study the folding mechanism of the human neuronal calcium sensor 1 (NCS1). Two intermediate structures induced by Ca2+ binding to the EF-hands were observed during refolding. The complete folding of the C domain is obligatory for the folding of the N domain, showing striking interdomain dependence. Molecular dynamics results reveal the atomistic details of the unfolding process and rationalize the different domain stabilities during mechanical unfolding. Through constant-force experiments and hidden Markov model analysis, the free energy landscape of the protein was reconstructed. Our results emphasize that NCS1 has evolved a remarkable complex interdomain cooperativity and a fundamentally different folding mechanism compared to structurally related proteins. (literal)

Prodotto di

• Nanobiosistemi (MD.P06.010.002) (Modulo)

Autore CNR

• LUCA BELLUCCI (Persona)
• CIRO CECCONI (Persona)
• ROSA DI FELICE (Persona)
• STEFANO CORNI (Persona)

Incoming links:

Autore CNR di

• ROSA DI FELICE (Persona)
• STEFANO CORNI (Persona)
• LUCA BELLUCCI (Persona)
• CIRO CECCONI (Persona)

Prodotto

• Nanobiosistemi (MD.P06.010.002) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Structure (Rivista)