alpha-Rhamnosidase activity in the marine isolate Novosphingobium sp PP1Y and its use in the bioconversion of flavonoids (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• alpha-Rhamnosidase activity in the marine isolate Novosphingobium sp PP1Y and its use in the bioconversion of flavonoids (Articolo in rivista) (literal)

Anno

• 2014-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1016/j.molcatb.2014.04.002 (literal)

Alternative label

• Izzo, Viviana; Tedesco, Pietro; Notomista, Eugenio; Pagnotta, Eduardo; Di Donato, Alberto; Trincone, Antonio; Tramice, Annabella (2014)
  alpha-Rhamnosidase activity in the marine isolate Novosphingobium sp PP1Y and its use in the bioconversion of flavonoids
  in Journal of molecular catalysis. B, Enzymatic (Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Izzo, Viviana; Tedesco, Pietro; Notomista, Eugenio; Pagnotta, Eduardo; Di Donato, Alberto; Trincone, Antonio; Tramice, Annabella (literal)

Pagina inizio

• 95 (literal)

Pagina fine

• 103 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 105 (literal)

Rivista

• Journal of molecular catalysis. B, Enzymatic (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 9 (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• University of Naples Federico II; Consiglio Nazionale delle Ricerche (CNR); University of Salerno , ICB-CNR (literal)

Titolo

• alpha-Rhamnosidase activity in the marine isolate Novosphingobium sp PP1Y and its use in the bioconversion of flavonoids (literal)

Abstract

• Crude protein extracts of Novosphingobium sp. PP1Y, a microorganism isolated from polluted marine waters in Pozzuoli (Italy), were analyzed for the presence of glycosidase activities. Particular attention was devoted to a alpha-L-rhamnosidase activity able to hydrolyze several flavonoids of interest for the pharmaceutical and food industries. This activity had an alkaline pH optimum and a moderate tolerance to the presence of organic solvents, appealing features for its possible biotechnological uses. An increase of the a-L-rhamnosidase activity in PPI V crude extracts was induced by adding naringin to the growth medium, suggesting the possibility to use material from Citrus industrial waste to induce the glycosidase activity expressed by strain PPlY and produce simultaneously high-added-value molecules from the hydrolysis of their flavonoids. In order to investigate on the enzymatic mechanism of PPlY a-L-rhamnosidase activity, hydrolysis products of PNP-alpha-L-rhamnopyranoside were analyzed by H-1-NMR experiments. The kinetic behaviour clearly indicated an inverting mechanism of hydrolysis for this novel enzymatic activity. (C) 2014 Elsevier B.V. All rights reserved. (literal)

Prodotto di

• Istituto di chimica biomolecolare (ICB) (Istituto)

Autore CNR

• EDUARDO PAGNOTTA (Unità di personale interno)
• ANTONIO TRINCONE (Persona)
• ANNABELLA TRAMICE (Unità di personale interno)

Insieme di parole chiave

• Keywords of "alpha-Rhamnosidase activity in the marine isolate Novosphingobium sp PP1Y and its use in the bioconversion of flavonoids" (Insieme di parole chiave)

Incoming links:

Autore CNR di

• EDUARDO PAGNOTTA (Unità di personale interno)
• ANTONIO TRINCONE (Persona)
• ANNABELLA TRAMICE (Unità di personale interno)

Prodotto

• Istituto di chimica biomolecolare (ICB) (Istituto)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Journal of molecular catalysis. B, Enzymatic (Rivista)

Insieme di parole chiave di

• Keywords of "alpha-Rhamnosidase activity in the marine isolate Novosphingobium sp PP1Y and its use in the bioconversion of flavonoids" (Insieme di parole chiave)