http://www.cnr.it/ontology/cnr/individuo/prodotto/ID312551

Drug-induced cellular death dynamics monitored by a highly sensitive organic electrochemical system (Articolo in rivista)

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Drug-induced cellular death dynamics monitored by a highly sensitive organic electrochemical system (Articolo in rivista) (literal)

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Agostino Romeo a, Giuseppe Tarabella a, Pasquale D'Angelo a, Cristina Caffarra b, Daniele Cretella b, Roberta Alfieri b, Piergiorgio Petronini b, Salvatore Iannotta a (2015)
Drug-induced cellular death dynamics monitored by a highly sensitive organic electrochemical system
in Biosensors & bioelectronics; Elsevier Science Publishers B.V., North Holland (Paesi Bassi)
(literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

Agostino Romeo a, Giuseppe Tarabella a, Pasquale D'Angelo a, Cristina Caffarra b, Daniele Cretella b, Roberta Alfieri b, Piergiorgio Petronini b, Salvatore Iannotta a (literal)

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a Institute of Materials for Electronics and Magnetism, National Research Council, Parco Area delle Scienze 37/A, Parma 43124, Italy b Unit of Experimental Oncology, Department of Clinical and Experimental Medicine, University of Parma, Parma 43125, Italy (literal)

Titolo

Drug-induced cellular death dynamics monitored by a highly sensitive organic electrochemical system (literal)

Abstract

We propose and demonstrate a sensitive diagnostic device based on an Organic Electrochemical Transistors (OECT) for direct in-vitro monitoring cell death. The system efficiently monitors cell death dynamics, being able to detect signals related to specific death mechanisms, namely necrosis or early/late apoptosis, demonstrating a reproducible correlation between the OECT electrical response and the trends of standard cell death assays. The innovative design of the Twell-OECT system has been modeled to better correlate electrical signals with cell death dynamics. To qualify the device, we used a human lung adenocarcinoma cell line (A549) that was cultivated on the micro-porous membrane of a Transwell (Twell) support, and exposed to the anticancer drug doxorubicin. Time-dependent and dose-dependent dynamics of A549 cells exposed to doxorubicin are evaluated by monitoring cell death upon exposure to a range of doses and times that fully covers the protocols used in cancer treatment. The demonstrated ability to direct monitor cell stress and death dynamics upon drug exposure using simple electronic devices and, possibly, achieving selectivity to different cell dynamics is of great interest for several application fields, including toxicology, pharmacology, and therapeutics. (literal)

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