Enzyme-responsive multifunctional surfaces for controlled uptake/release of (bio)molecules (Articolo in rivista)

Type
Label
  • Enzyme-responsive multifunctional surfaces for controlled uptake/release of (bio)molecules (Articolo in rivista) (literal)
Anno
  • 2014-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1016/j.colsurfb.2014.08.034 (literal)
Alternative label
  • Mortato M.; Argentiere S.; De Gregorio G.L.; Gigli G.; Blasi L. (2014)
    Enzyme-responsive multifunctional surfaces for controlled uptake/release of (bio)molecules
    in Colloids and surfaces. B, Biointerfaces (Online)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Mortato M.; Argentiere S.; De Gregorio G.L.; Gigli G.; Blasi L. (literal)
Pagina inizio
  • 89 (literal)
Pagina fine
  • 95 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url
  • http://www.scopus.com/inward/record.url?eid=2-s2.0-84915756134&partnerID=q2rCbXpz (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 123 (literal)
Rivista
Note
  • Scopu (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • University of Salento, Superior School ISUFI, Arnesano, Lecce, I-73100, Italy; CNR-Institute of Nanoscience, NNL-Lecce, Via Arnesano, Lecce, I-73100, Italy; Fondazione Filarete, Viale Ortles 22/4, Milano, 20139, Italy; Center for Biomolecular Nanotechnologies - Fondazione Istituto Italiano di Tecnologia, Via Barsanti, Arnesano Lecce, 73010, Italy (literal)
Titolo
  • Enzyme-responsive multifunctional surfaces for controlled uptake/release of (bio)molecules (literal)
Abstract
  • The current trend in the development of biomaterials is towards bioactive and biodegradable systems. In particular, enzyme-responsive structures are useful tools to realize biodegradable surfaces for the controlled delivery of biomolecules/drugs through a triggered surface erosion process. Up to now, enzyme-responsive structures have been designed by covalent linkage between synthetic polymers and biodegradable functionalities that are responsive to chemical and biological cues (i.e. proteases or pH) [1-4].Here, we present a novel approach to achieve enzyme-responsive surface-attached networks by exploiting the non-covalent interaction between streptavidin and biotin. The functional component of this three-dimensional (3D) structure is a layer of biotinylated peptides that are degraded by the action of specific proteases.The system was stable under typical physiological conditions; however, it was efficiently degraded upon enzyme exposure. Further, the controlled release of biomolecules and drugs - previously entrapped into the surface-attached network - was demonstrated to occur as a consequence of the enzymatic cleavage.This versatile approach does not require complex chemical procedures. Interestingly, it can be easily adapted to different enzyme-peptide partners and therefore is very attractive for tissue replacement, drug delivery and biosensing. (literal)
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