Two interconvertible folds modulate the activity of a DNA aptamer against transferrin receptor (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Two interconvertible folds modulate the activity of a DNA aptamer against transferrin receptor (Articolo in rivista) (literal)

Anno

• 2014-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1038/mtna.2013.71 (literal)

Alternative label

• Porciani D.[ 1,2,3 ]; Signore G.[ 3 ]; Marchetti L.[ 1,2 ]; Mereghetti P.[ 3 ]; Nifosi R.[ 1,2 ]; Beltram F.[ 1,2 ] (2014)
  Two interconvertible folds modulate the activity of a DNA aptamer against transferrin receptor
  in MOLECULAR THERAPY-NUCLEIC ACIDS
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Porciani D.[ 1,2,3 ]; Signore G.[ 3 ]; Marchetti L.[ 1,2 ]; Mereghetti P.[ 3 ]; Nifosi R.[ 1,2 ]; Beltram F.[ 1,2 ] (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url

• http://www.scopus.com/inward/record.url?eid=2-s2.0-84893054594&partnerID=q2rCbXpz (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 3 (literal)

Rivista

• MOLECULAR THERAPY-NUCLEIC ACIDS (Rivista)

Note

• Scopu (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• [ 1 ] Scuola Normale Super Pisa, NEST, Pisa, Italy [ 2 ] CNR, Ist Nanosci, I-56100 Pisa, Italy [ 3 ] Ist Italiano Tecnol, Ctr Nanotechnol Innovat NEST, Pisa, Italy (literal)

Titolo

• Two interconvertible folds modulate the activity of a DNA aptamer against transferrin receptor (literal)

Abstract

• Thanks to their ability to recognize biomolecular targets with high affinity and specificity, nucleic acid aptamers are increasingly investigated as diagnostic and therapeutic tools, particularly when their targets are cell-surface receptors. Here, we investigate the relationship between the folding of an anti-mouse transferrin receptor DNA aptamer and its interaction with the transferrin receptor both in vitro and in living cells. We identified and purified two aptamer conformers by means of chromatographic techniques. Fluorescence-anisotropy measurements showed that only one fold is able to bind mouse transferrin receptor. Besides displaying enhanced endocytosis in living mouse fibroblasts, the purified active fold is internalized also in human pancreatic cancer cells. Starting from these observations, we rationally designed variations of the parent sequence aimed at stabilizing the active fold, and consequently increase aptamer activity. A truncated version and full-length mutants with higher affinity than the parent sequence are shown. © 2014 The American Society of Gene & Cell Therapy. (literal)

Prodotto di

• Fenomeni coerenti in nanosistemi semiconduttori ed ibridi (MD.P06.021.002) (Modulo)
• Progettazione ed applicazioni di Nanobiotecnologie (MD.P06.022.002) (Modulo)

Autore CNR

• RICCARDO NIFOSI' (Unità di personale interno)
• FABIO BELTRAM (Persona)
• LAURA MARCHETTI (Unità di personale esterno)

Insieme di parole chiave

• Parole chiave di "Two interconvertible folds modulate the activity of a DNA aptamer against transferrin receptor" (Insieme di parole chiave)

Incoming links:

Autore CNR di

• RICCARDO NIFOSI' (Unità di personale interno)
• FABIO BELTRAM (Persona)
• LAURA MARCHETTI (Unità di personale esterno)

Prodotto

• Fenomeni coerenti in nanosistemi semiconduttori ed ibridi (MD.P06.021.002) (Modulo)
• Progettazione ed applicazioni di Nanobiotecnologie (MD.P06.022.002) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• MOLECULAR THERAPY-NUCLEIC ACIDS (Rivista)

Insieme di parole chiave di

• Parole chiave di "Two interconvertible folds modulate the activity of a DNA aptamer against transferrin receptor" (Insieme di parole chiave)