Fullerol in human lens and retinal pigment epithelial cells: time domain fluorescence spectroscopy and imaging (Articolo in rivista)

Type
Label
  • Fullerol in human lens and retinal pigment epithelial cells: time domain fluorescence spectroscopy and imaging (Articolo in rivista) (literal)
Anno
  • 2011-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1039/c0pp00312c (literal)
Alternative label
  • Taroni, Paola; D'Andrea, Cosimo; Valentini, Gianluca; Cubeddu, Rinaldo; Hu, Dan-Ning; Roberts, Joan E. (2011)
    Fullerol in human lens and retinal pigment epithelial cells: time domain fluorescence spectroscopy and imaging
    in Photochemical & photobiological sciences (Print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Taroni, Paola; D'Andrea, Cosimo; Valentini, Gianluca; Cubeddu, Rinaldo; Hu, Dan-Ning; Roberts, Joan E. (literal)
Pagina inizio
  • 904 (literal)
Pagina fine
  • 910 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 10 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali
  • 7 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 6 (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Polytechnic University of Milan; Consiglio Nazionale delle Ricerche (CNR); Istituto Italiano di Tecnologia - IIT; New York Eye & Ear Infirm; Fordham University (literal)
Titolo
  • Fullerol in human lens and retinal pigment epithelial cells: time domain fluorescence spectroscopy and imaging (literal)
Abstract
  • Fullerol is a fullerene derivative that is extensively hydroxylated [nano-C(60)(OH)(24)] and this makes it water-soluble. These fullerene derivatives have shown promise as drug carriers that bypass ocular barriers but fullerols are also potentially phototoxic to human lens and retinal tissues. Fluorescence imaging is a powerful and non-invasive means of probing nanoparticles in biological systems. However, fullerol nanoparticles have a very low level of fluorescence and have not as yet been imaged in vitro and in vivo. Using specialized measurements including time-correlated single photon counting (TCSPC), fullerol fluorescence was determined in aqueous solutions and detected in both human lens and retinal pigment epithelial cells. Time-resolved fluorescence of fullerol (5-200 mu M) was characterized in aqueous environment, where the fluorescence decay is best fitted with three lifetimes (3 ns, 0.7-0.9 ns and 0.2 ns). Time-resolved microspectrofluorimetry and time-gated fluorescence imaging were performed on both human lens and retinal pigment epithelial cells incubated with increasing fullerol doses (5-500 mu M and 5-50 mu M, respectively). Upon increasing concentration, we observe some shortening of the lifetimes, a reduction in the relative amplitude of the shortest-living component and a corresponding increase in the weight of the intermediate-living species. Time-gated imaging of fullerol fluorescence provided information on its intracellular distribution that correlates with progressive cell damage. Therefore time-gated imaging may potentially be used as a means to investigate fullerol distribution and toxicity in the human lens and retina in vivo. (literal)
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