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A novel bipartite launch system for a potexviral vector suitable for either protein expression or virus-induced gene silencing (VIGS) (Abstract/Comunicazione in atti di convegno)

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A novel bipartite launch system for a potexviral vector suitable for either protein expression or virus-induced gene silencing (VIGS) (Abstract/Comunicazione in atti di convegno) (literal)

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Lim H., Vaira A., Domier L.L., Hammond J. (2010)
A novel bipartite launch system for a potexviral vector suitable for either protein expression or virus-induced gene silencing (VIGS)
in APS Annual meeting, Charlotte, NC, USA, august 7-11 2010
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Lim H., Vaira A.M., Hammond J. - USDA ARS MPPL, Beltsville, MD, U.S.A. Domier L.L. - USDA ARS, Urbana, IL, U.S.A. (literal)

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A novel bipartite launch system for a potexviral vector suitable for either protein expression or virus-induced gene silencing (VIGS) (literal)

Abstract

We have developed plant virus-based vectors for virus-induced gene silencing (VIGS) and protein expression, based on Alternanthera mosaic virus (AltMV), for infection of a wide range of host plants including Nicotiana benthamiana, Arabidopsis thaliana, and Glycine max (soybean). Infection may be established by either mechanical inoculation of in vitro transcripts or via agroinfiltration. In vivo transcripts produced by co-agroinfiltration of bacteriophage T7 RNA polymerase resulted in T7-driven AltMV infection from a binary vector in the absence of the Cauliflower mosaic virus 35S promoter. An artificial bipartite viral vector delivery system was created by separating the AltMV RNA-dependent RNA polymerase and Triple Gene Block (TGB)123-Coat protein (CP) coding regions into two constructs each bearing the AltMV 5´ and 3´ non-coding regions, which recombined in planta to generate a full-length AltMV genome. Substitution of TGB1 L(88)P, and equivalent changes in other potexvirus TGB1 proteins, affected RNA silencing suppression efficacy and suitability of the vectors from protein expression to VIGS. (literal)

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