http://www.cnr.it/ontology/cnr/individuo/prodotto/ID308919
Morphological characterisation and diagnostics of Xiphinema non-americanum group species (Nematoda: Longidoridae) from Romania using mutiplex PCR (Articolo in rivista)
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- Morphological characterisation and diagnostics of Xiphinema non-americanum group species (Nematoda: Longidoridae) from Romania using mutiplex PCR (Articolo in rivista) (literal)
- Anno
- 2013-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.2478/s11687-013-0133-3 (literal)
- Alternative label
Groza M., Lazarova S., Costache C., De Luca F., Rosca I., Fanelli E., Peneva V. K. (2013)
Morphological characterisation and diagnostics of Xiphinema non-americanum group species (Nematoda: Longidoridae) from Romania using mutiplex PCR
in Helminthologia (Tlacené vyd.)
(literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Groza M., Lazarova S., Costache C., De Luca F., Rosca I., Fanelli E., Peneva V. K. (literal)
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- http://www.degruyter.com/view/j/helmin.2013.50.issue-3/s11687-013-0133-3/s11687-013-0133-3.xml (literal)
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- GROZA M., ROSCA I.-Univ Agron Sci & Vet Med Bucharest, Bucharest 011464, Romania
LAZAROVA S., PENEVA V. K.-Bulgarian Acad Sci, Inst Biodivers & Ecosyst Res, BU-1113 Sofia, Bulgaria
GROZA M., COSTACHE C.- Cent Phytosanit Lab, Voluntari 077190, Ilfov, Romania (literal)
- Titolo
- Morphological characterisation and diagnostics of Xiphinema non-americanum group species (Nematoda: Longidoridae) from Romania using mutiplex PCR (literal)
- Abstract
- Additional data on the occurrence and distribution of Xiphinema non-americanum group species in Romania are provided. Xiphinema diversicaudatum, X. index, X. vuittenezi and X. italiae were recovered from vineyards and cherry fruit trees; adults and juvenile stages were described and analysed and the morphology/variability discussed. Multiplex PCR diagnostic test using species-specific primers designed by Wang et al. (2003) yielded amplification products with expected lengths for all screened populations of these four species. Two ribosomal markers (D2-D3 28 LSU rDNA and ITS) were sequenced and ITS RFLP patterns were obtained from two X. vuittenezi populations, which have shown some morphological differences. Comparatively low level of interpopulation genetic dissimilarity (<1 %) was revealed for both markers (for D2D3 - 0.5 %; for ITS - 0.7 %). Both populations of X. vuittenezi studied produced identical ITS-RFLP specific pattern that clearly identify this species. (literal)
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