Candidate genes for engineering plants degrading polycyclic aromatic hydrocarbons (Abstract/Poster in atti di convegno)

Type
Label
  • Candidate genes for engineering plants degrading polycyclic aromatic hydrocarbons (Abstract/Poster in atti di convegno) (literal)
Anno
  • 2012-01-01T00:00:00+01:00 (literal)
Alternative label
  • Di Matteo A., Monti M.M., Pedata P.A., Van Der Lee T.A.J., Rao M.A., Testa A. (2012)
    Candidate genes for engineering plants degrading polycyclic aromatic hydrocarbons
    in 56th Italian Society of Agricultural Genetics Annual Congress, Perugia, 17-20 settembre 2012
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Di Matteo A., Monti M.M., Pedata P.A., Van Der Lee T.A.J., Rao M.A., Testa A. (literal)
Pagina inizio
  • 7.60 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#titoloVolume
  • Proceedings of the 56th Italian Society of Agricultural Genetics Annual Congress Perugia (literal)
Note
  • Abstract (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Di Matteo A., Rao M. A., Testa A. - Università degli Studi di Napoli Federico II, Dip. Agraria Van Der Lee T. A. J. Wageningen UR, Plant Research International, Department Bio-Interactions and Plant Health, The Netherlands (literal)
Titolo
  • Candidate genes for engineering plants degrading polycyclic aromatic hydrocarbons (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#isbn
  • 978-88-904570-1-2 (literal)
Abstract
  • Polycyclic aromatic hydrocarbons (PAHs) are a class of several hundred individual compounds containing at least two condensed aromatic rings that are known to be toxic, mutagenic, carcinogenic and teratogenic. They are common pollutants in the environment reaching the highest levels of contamination especially within disused industrial sites. They have a low intrinsic biochemistry biodegradability determined mainly by their strong hydrophobicity thus bioaccumulate in the environment and propagate to trophic levels of the food chain till humans. Nevertheless, plants and micro-organisms can operate degradation of PAHs. By mean of their enzymes they are able to modify extensively the structure or to degrade the organic molecule into harmless inorganic end products. The aim of this project was to identify genes and pathways involved in PAHs degrading processes and to exploit them for engineering plants with enhanced bioremediation ability. Recently, we isolated a number of indigenous fungal species from soil contaminated with PAHs, we introduced them in a collection and catalogued them according to their ISSR profile. Among all, a Byssoclamis nivea strain showed a conspicuous degrading activity on PHAs when added to the culture medium. DNA and RNA sequence at genomic level has not been provided so far in B. nivea thus tremendously limiting biotechnological approaches in this specie. For this reason, we produced a normalized 3'-fragment cDNA library from mycelia grown in different condition of media composition, pH, temperature, light intensity and PHA supplementation. This library has been sequenced by 454 FLX technology and the assembling procedure produced 34880 contigs and 88400 singletons. Among all, a number mRNAs annotated as glutathione S-transferases, P450 monoxygenases and dioxygenases showed increased expression when PAHs were supplemented in the media. These genes have been selected for their expression in the yeast Yarrowia lipolytica and tobacco plants. Further functional characterization of candidate genes will provide additional insights on molecular mechanisms and processes involved in detoxifying hazardous contaminants. Also, characterization of engineered yeasts and plants is expected to provide additional cues in implementing new systems for remediation, in a confined environment, of soil and contaminated waters. (literal)
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