Droplet microfluidics platform for highly sensitive and quantitative detection of malaria-causing plasmodium parasites based on enzyme activity measurement (Articolo in rivista)

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  • Droplet microfluidics platform for highly sensitive and quantitative detection of malaria-causing plasmodium parasites based on enzyme activity measurement (Articolo in rivista) (literal)
Anno
  • 2012-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1021/nn3038594 (literal)
Alternative label
  • Juul S.; Nielsen C.J.F.; Labouriau R.; Roy A.; Tesauro C.; Jensen P.W.; Harmsen C.; Kristoffersen E.L.; Chiu Y.-L.; Frohlich R.; Fiorani P.; Cox-Singh J.; Tordrup D.; Koch J.; Bienvenu A.-L.; Desideri A.; Picot S.; Petersen E.; Leong K.W.; Ho Y.-P.; Stougaard M.; Knudsen B.R. (2012)
    Droplet microfluidics platform for highly sensitive and quantitative detection of malaria-causing plasmodium parasites based on enzyme activity measurement
    in ACS nano
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Juul S.; Nielsen C.J.F.; Labouriau R.; Roy A.; Tesauro C.; Jensen P.W.; Harmsen C.; Kristoffersen E.L.; Chiu Y.-L.; Frohlich R.; Fiorani P.; Cox-Singh J.; Tordrup D.; Koch J.; Bienvenu A.-L.; Desideri A.; Picot S.; Petersen E.; Leong K.W.; Ho Y.-P.; Stougaard M.; Knudsen B.R. (literal)
Pagina inizio
  • 10676 (literal)
Pagina fine
  • 10683 (literal)
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  • http://www.scopus.com/inward/record.url?eid=2-s2.0-84871597688&partnerID=q2rCbXpz (literal)
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  • 6 (literal)
Rivista
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  • 12 (literal)
Note
  • Scopu (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Department of Biomedical Engineering, Duke University, Durham, NC, United States; Department of Molecular Biology and Genetics, Aarhus University, Denmark; Department of Biology, University of Rome Tor Vergata, Via della Ricerca Scientifica 1, 00133 Rome, Italy; Institute of Translational Pharmacology, National Research Council, CNR, Rome, Italy; School of Medicine, University of St Andrews, Fife KY16 9TF, United Kingdom; Department of Pathology, Aarhus University Hospital, Denmark; Malaria Research Unit, SMITH, Hospices Civils de Lyon, Lyon, France; Department of Infectious Diseases, Institute of Clinical Medicine, Aarhus University Hospital-Skejby, Denmark; Interdisciplinary Nanoscience Center (INANO), Aarhus University, Denmark; Malaria Research Centre, University Malaysia Sarawak, Kuching, Sarawak, Malaysia (literal)
Titolo
  • Droplet microfluidics platform for highly sensitive and quantitative detection of malaria-causing plasmodium parasites based on enzyme activity measurement (literal)
Abstract
  • We present an attractive new system for the specific and sensitive detection of the malaria-causing Plasmodium parasites. The system relies on isothermal conversion of single DNA cleavage-ligation events catalyzed specifically by the Plasmodium enzyme topoisomerase I to micrometer-sized products detectable at the single-molecule level. Combined with a droplet microfluidics lab-on-a-chip platform, this design allowed for sensitive, specific, and quantitative detection of all human-malaria-causing Plasmodium species in single drops of unprocessed blood with a detection limit of less than one parasite/?L. Moreover, the setup allowed for detection of Plasmodium parasites in noninvasive saliva samples from infected patients. During recent years malaria transmission has declined worldwide, and with this the number of patients with low-parasite density has increased. Consequently, the need for accurate detection of even a few parasites is becoming increasingly important for the continued combat against the disease. We believe that the presented droplet microfluidics platform, which has a high potential for adaptation to point-of-care setups suitable for low-resource settings, may contribute significantly to meet this demand. Moreover, potential future adaptation of the presented setup for the detection of other microorganisms may form the basis for the development of a more generic platform for diagnosis, fresh water or food quality control, or other purposes within applied or basic science. © 2012 American Chemical Society. (literal)
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