http://www.cnr.it/ontology/cnr/individuo/prodotto/ID305952
G-quadruplex DNA recognition by nucleophosmin: New insights from protein dissection (Articolo in rivista)
- Type
- Label
- G-quadruplex DNA recognition by nucleophosmin: New insights from protein dissection (Articolo in rivista) (literal)
- Anno
- 2014-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1016/j.bbagen.2014.02.017 (literal)
- Alternative label
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Scognamiglio P.L.; Di Natale C.; Leone M.; Poletto M.; Vitagliano L.; Tell G.; Marasco D. (literal)
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- Department of Pharmacy, University of Naples Federico II, DFM-Scarl, 80134 Naples, Italy; CIRPEB: Centro Interuniversitario di Ricerca sui Peptidi Bioattivi, University of Naples Federico II, DFM-Scarl, 80134 Naples, Italy; Center for Advanced Biomaterials for Healthcare CRIB, Istituto Italiano di Tecnologia (IIT), 80125 Naples, Italy; Institute of Biostructures and Bioimaging, CNR, 80134 Naples, Italy; Department of Medical and Biological Sciences, University of Udine, 33100 Udine, Italy (literal)
- Titolo
- G-quadruplex DNA recognition by nucleophosmin: New insights from protein dissection (literal)
- Abstract
- Nucleophosmin (NPM1, B23) is a multifunctional protein that is involved in a variety of fundamental biological processes. NPM1/B23 deregulation is implicated in the pathogenesis of several human malignancies. This protein exerts its functions through the interaction with a multiplicity of biological partners. Very recently it is has been shown that NPM1/B23 specifically recognizes DNA G-quadruplexes through its C-terminal region. Methods Through a rational dissection approach of protein here we show that the intrinsically unfolded regions of NPM1/B23 significantly contribute to the binding of c-MYC G-quadruplex motif. Interestingly, the analysis of the ability of distinct NPM1/B23 fragments to bind this quadruplex led to the identifications of distinct NPM1/B23-based peptides that individually present a high affinity for this motif. Results These results suggest that the tight binding of NPM1/B23 to the G-quadruplex is achieved through the cooperation of both folded and unfolded regions that are individually able to bind it. The dissection of NPM1/B23 also unveils that its H1 helix is intrinsically endowed with an unusual thermal stability. Conclusions These findings have implications for the unfolding mechanism of NPM1/B23, for the G-quadruplex affinity of the different NPM1/B23 isoforms and for the design of peptide-based molecules able to interact with this DNA motif. General observation This study sheds new light in the molecular mechanism of the complex NPM1/G-quadruplex involved in acute myeloid leukemia (AML) disease. © 2014 Elsevier B.V. All rights reserved. (literal)
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