Human placental derived stem cells grown on a synthetic-fibrin scaffold for cardiac tissue engineering (Abstract/Poster in rivista)

Type
Label
  • Human placental derived stem cells grown on a synthetic-fibrin scaffold for cardiac tissue engineering (Abstract/Poster in rivista) (literal)
Anno
  • 2013-01-01T00:00:00+01:00 (literal)
Alternative label
  • Ledda, M.; Briganti, E.; Losi, P.; Grimaldi, S.; Marchese, R.; Soldani, G.; Lisi, A. (2013)
    Human placental derived stem cells grown on a synthetic-fibrin scaffold for cardiac tissue engineering
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Ledda, M.; Briganti, E.; Losi, P.; Grimaldi, S.; Marchese, R.; Soldani, G.; Lisi, A. (literal)
Pagina inizio
  • A114 (literal)
Pagina fine
  • A114 (literal)
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  • 24 (literal)
Rivista
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  • 1 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 12 (literal)
Note
  • ISI Web of Science (WOS) (literal)
  • Poster (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Consiglio Nazionale delle Ricerche (CNR); Consiglio Nazionale delle Ricerche (CNR); FBF S Peter Hosp (literal)
Titolo
  • Human placental derived stem cells grown on a synthetic-fibrin scaffold for cardiac tissue engineering (literal)
Abstract
  • Tissue-engineering strategies combining scaffolds and stem cells to enhance cardiac repair is considered a very promising approach for the treatment of damaged myocardium. For this goal a suitable cell delivery system to improve tissue regeneration, without affecting cardiac contractile function is required. For their multi-lineage differentiation potential and low immunogenic properties Mesenchymal Stem Cells (MSCs) isolated from term Amniotic Membrane (AM) are considered a very attractive source for cardiac repair. In this study an elastic scaffold, obtained combining a fibrin layer with a poly(ether)urethanepolydimethylsiloxane (PEtU-PDMS) layer, was used as a substrate for ''in vitro'' growth and differentiation of human amniotic mesenchymal stromal cell (hAMSCs). Cell proliferation and metabolic activity of these cells, grown on Combined Synthetic-Fibrin scaffold, were evaluated by bromodeoxyuridine and WST-1 assays for up to 14 days of culture. Their cardio myogenic commitment was induced, for the first time in this cell population, by a nitric oxide (NO) treatment. The NO treated hAMSCs, showed morphological changes, an increase of the messenger cardiac differentiation markers [troponinI (TnI) and NK2 transcription factor related locus 5 (Nkx2.5)] and a modulation of the endothelial markers [vascular endothelial growth factor (VEGF) and kinase insert domain receptor (KDR)] by RT-PCR analysis. The results of this study propose a combined synthetic fibrin scaffold, able to support growth and cardio myogenic commitment of hAMSCs, as a new strategy that could be used in cardiac regenerative medicine. (literal)
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