http://www.cnr.it/ontology/cnr/individuo/prodotto/ID304695
Cytological mechanism of Cardinium-induced cytoplasmic incompatibility in Encarsia pergandiella (Hymenoptera: Aphelinidae): preliminary results. (Abstract/Poster in atti di convegno)
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- Label
- Cytological mechanism of Cardinium-induced cytoplasmic incompatibility in Encarsia pergandiella (Hymenoptera: Aphelinidae): preliminary results. (Abstract/Poster in atti di convegno) (literal)
- Anno
- 2014-01-01T00:00:00+01:00 (literal)
- Alternative label
Gebiola M., Kelly S.E., Giorgini M., Hunter M.S. (2014)
Cytological mechanism of Cardinium-induced cytoplasmic incompatibility in Encarsia pergandiella (Hymenoptera: Aphelinidae): preliminary results.
in 8th International Wolbachia Conference, Igls, Innsbruck, Austria, 6-11 June 2014
(literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Gebiola M., Kelly S.E., Giorgini M., Hunter M.S. (literal)
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- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#titoloVolume
- Abstract Book of the 8th International Wolbachia Conference (literal)
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- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- Kelly S.E., Hunter M.S.: Hunter MS - University of Arizona, Department of Entomology, Tucson, AZ, USA (literal)
- Titolo
- Cytological mechanism of Cardinium-induced cytoplasmic incompatibility in Encarsia pergandiella (Hymenoptera: Aphelinidae): preliminary results. (literal)
- Abstract
- Cytoplasmic incompatibility (CI) between closely related populations occurs frequently in
arthropods as a consequence of infections by Wolbachia and Cardinium endosymbionts. CI
causes sterility in matings between infected males and uninfected or differently-infected
females. CI-bacteria infect a large number of arthropods and strongly influence the ecology
and evolution of their hosts. CI may also affect the invasiveness of alien genotypes and the
effectiveness of biocontrol agents. Cytological analyses of Wolbachia CI crosses in Drosophila
and Nasonia have provided insights into the mechanism of CI. These studies have shown
that incompatible embryos die in the first mitotic division of the zygote, and implicate
paternal chromatin remodeling and asynchrony of male and female pronuclei entering the
first mitotic division. The delay leads to abnormal division or the exclusion of male
chromatids. So far, nothing is known about the sequence of events in the embryogenesis of
incompatible CI Cardinium crosses.
We conducted a cytological analysis of reproductive incompatibility caused by CI Cardinium
in Encarsia pergandiella. We set up incompatible and control matings, dissected eggs from
whitefly hosts, and fixed and stained eggs with DAPI during early embryogenesis. DAPIstained
eggs were imaged with a confocal microscope. We also attempted live-staining with
the nucleic acid stain Syto-11 to allow for visualization of embryogenesis in real time. This
technique involves dissection in buffer followed by a short incubation before imaging with a
deconvolution microscope.
We observed the formation of chromatin bridges after the first mitotic division of the zygote,
which is a common feature of Wolbachia-induced CI. Our preliminary results suggest a
common mechanism of CI induced by the two bacterial lineages. Identifying parallels
between CI caused by Wolbachia and Cardinium will shed light on fundamental mechanisms
that underlie this reproductive incompatibility. (literal)
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