Surface expression and axon/dendritic distribution of mGlu5 receptors in hippocampal wild type and Fmr1 knockout neurons (Abstract/Poster in atti di convegno)

Type
Label
  • Surface expression and axon/dendritic distribution of mGlu5 receptors in hippocampal wild type and Fmr1 knockout neurons (Abstract/Poster in atti di convegno) (literal)
Anno
  • 2011-01-01T00:00:00+01:00 (literal)
Alternative label
  • Spatuzza, M.; D'Antoni, S.; Aloisi, E.; Catania, M. (2011)
    Surface expression and axon/dendritic distribution of mGlu5 receptors in hippocampal wild type and Fmr1 knockout neurons
    in 41st annual meeting of the Society for Neuroscience, Washington DC, U.S.A., 12-16/11/11
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Spatuzza, M.; D'Antoni, S.; Aloisi, E.; Catania, M. (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 41 (literal)
Note
  • Poster (literal)
  • SI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • 1Inst. of Neurolog. Sci. (ISN), Natl. Res. Council (CNR), Catania, Italy; 2PhD program in Neuropharmacology, Univ. of Catania, Catania, Italy; 3IRCCS Oasi Maria SS, Troina (EN), Italy (literal)
Titolo
  • Surface expression and axon/dendritic distribution of mGlu5 receptors in hippocampal wild type and Fmr1 knockout neurons (literal)
Abstract
  • Fragile X Syndrome (FXS) is a leading genetic cause of intellectual disability. It is caused by the lack of expression of Fragile X Mental Retardation Protein (FMRP), an RNA-binding protein involved in mRNA transport, stability and translation. A growing number of studies supports a role of group-I mGlu receptors (mGlu1 and mGlu5) in the pathophysiology of FXS. However, the expression of mGlu5 receptors have not been systematically investigated in FXS mouse model. In FXS a reduced number of mGlu5 receptors are associated to Homer proteins, a class of scaffolding proteins which anchor mGlu receptors to post-synaptic density (Giuffrida et al., J Neurosci., 2005). Considering that mGlu5-Homer interaction regulates the surface expression and axonal/dendritic distribution of mGlu5 receptors (Ango et al., J Neurosci., 2000; Mol Cell Neurosci 2002), we hypothesised that mGlu5 receptors might be differently targeted to cell surface of Fmr1 knock out (KO) neurons. We studied the expression of mGlu5 receptors in hippocampal synaptic proteins prepared from mice of different ages by Western blot analysis and the surface expression of mGlu5 receptors in hippocampal cultured neurons by confocal analysis of surface fluorescence-labeled receptors (kind gift of Prof. Shigemoto). Hippocampal cell cultures were prepared from wild type (WT) and Fmr1 KO newborn mice (P0-P1) and immunocytochemistry was performed at different days in vitro (3, 7, 12 DIV). Double- or triple-labelling experiments with anti-MAP2 and/or anti-tau1 antibodies were carried out to visualize the presence of mGlu5 receptors in dendrites and axons, respectively. Our results show that: a) mGlu5 receptors are more expressed in hippocampal synaptic proteins at postnatal day (P) 21 and 45, but not at 15 month of age; b) mGlu5 receptors are more expressed on the cell surface in Fmr1 KO than in WT cultured neurons. Interestingly, mGlu5 receptors were clearly detectable in the cellular body and dendrites in WT neurons, whereas in Fmr1 KO neurons a strong staining was also observed in the axons. Our data suggest that mGlu5 receptors are differently targeted to the cell surface and differently distributed in dendrites and axons in WT and Fmr1 KO mice. (literal)
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