A nano-LC/UV method for the analysis of principal phenolic compounds in commercial citrus juices and evaluation of antioxidant potential. (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• A nano-LC/UV method for the analysis of principal phenolic compounds in commercial citrus juices and evaluation of antioxidant potential. (Articolo in rivista) (literal)

Anno

• 2014-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1002/elps.201300621 (literal)

Alternative label

• A. Rocco, C. Fanali, L.Dugo, L. Mondello (2014)
  A nano-LC/UV method for the analysis of principal phenolic compounds in commercial citrus juices and evaluation of antioxidant potential.
  in Electrophoresis (Weinh., Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• A. Rocco, C. Fanali, L.Dugo, L. Mondello (literal)

Pagina inizio

• 1701 (literal)

Pagina fine

• 1708 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 35 (literal)

Rivista

• Electrophoresis (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 8 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 11 (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Istituto di Metodologie Chimiche (IMC) Consiglio Nazionale delle Ricerche. Università Campus Bio-Medico - Roma, Italia Università di Messina (literal)

Titolo

• A nano-LC/UV method for the analysis of principal phenolic compounds in commercial citrus juices and evaluation of antioxidant potential. (literal)

Abstract

• In this study, a simple and rapid methodology to analyze and quantify principal flavanones in citrus fruit juices through the use of a nano-LC/UV-Vis apparatus, employing a 75m id capillary column packed with sub-2m particles C18 stationary phase for 10 cm, was developed. All compounds were baseline resolved working with a step gradient elution mode in 10min. The developed analytical method was validated and the resulting RSD% for intra- and interday repeatability, related to retention time and peak area, were <4.7 and 5.5%, respectively. LOD and LOQ values corresponded to 0.40 and 1.56g/mL for didymin, hesperitin, and narinegenin, while for the other flavanones were 0.78 and 3g/mL, respectively. Good linearity with acceptable determination coefficients R2 was obtained in the range between LOQ concentration and 200g/mL (500g/mL naringin and hesperidin). Good recovery values were also obtained. Then, the method was applied to the analysis of selected hand-squeezed and commercial citrus juices. Further, the nano-LC system was coupled to a mass spectrometer to confirm analyte identification. Antioxidant capacity of selected samples was also evaluated measured by Folin-Ciocalteu assay and Trolox equivalent antioxidant capacity assay. Results were compared to determine total flavanones concentrations. (literal)

Prodotto di

• Sviluppo di metodologie chimiche avanzate per lo studio di fenomeni delle scienze omiche (PM.P06.019.001) (Modulo)
• Istituto di metodologie chimiche (IMC) (Istituto)

Autore CNR

• ANNA ROCCO (Unità di personale interno)

Insieme di parole chiave

• Keywords of "A nano-LC/UV method for the analysis of principal phenolic compounds in commercial citrus juices and evaluation of antioxidant potential." (Insieme di parole chiave)

Incoming links:

Prodotto

• Istituto di metodologie chimiche (IMC) (Istituto)
• Sviluppo di metodologie chimiche avanzate per lo studio di fenomeni delle scienze omiche (PM.P06.019.001) (Modulo)

Autore CNR di

• ANNA ROCCO (Unità di personale interno)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Electrophoresis (Rivista)

Insieme di parole chiave di

• Keywords of "A nano-LC/UV method for the analysis of principal phenolic compounds in commercial citrus juices and evaluation of antioxidant potential." (Insieme di parole chiave)