Influence of HeiferPlusTM treatment on some functional parameters of frozen-thawed bovine spermatozoa assessed by flow cytometry. (Abstract/Poster in convegno)

Type

• Abstract/Poster in convegno (Classe)
• Prodotto della ricerca (Classe)

Label

• Influence of HeiferPlusTM treatment on some functional parameters of frozen-thawed bovine spermatozoa assessed by flow cytometry. (Abstract/Poster in convegno) (literal)

Anno

• 2013-01-01T00:00:00+01:00 (literal)

Alternative label

• Guastamacchia Rosangela1 , Minervini Fiorenza2 , Garbetta Antonella2,Vittoria Lucia Barile1 (2013)
  Influence of HeiferPlusTM treatment on some functional parameters of frozen-thawed bovine spermatozoa assessed by flow cytometry.
  in 17° European Society for Domestic Animal Reproduction (ESDAR), Bologna, 12-14 september 2013
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Guastamacchia Rosangela1 , Minervini Fiorenza2 , Garbetta Antonella2,Vittoria Lucia Barile1 (literal)

Pagina inizio

• 83 (literal)

Pagina fine

• 83 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#volumeInCollana

• 48/suppl 1 (literal)

Rivista

• Reproduction in domestic animals (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 1 (literal)

Note

• Poster (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• 1 Consiglio per la Ricerca e la sperimentazione in Agricoltura, Animal Production Research Centre (CRA-PCM), Monterotondo (Roma), Italy 2 Institute of Sciences of Food Production (ISPA), CNR, Bari (Italy) (literal)

Titolo

• Influence of HeiferPlusTM treatment on some functional parameters of frozen-thawed bovine spermatozoa assessed by flow cytometry. (literal)

Abstract

• HeiferPlusTM is a new sperm sexing agent used to increase the percentage of heifer calves in dairy and beef cattle by enhancing the fertility and the motility of the X-chromosome bearing sperm. The aim of this study was to assess the influence of HeiferPlusTM treatment on sperm functional parameters of frozen-thawed bovine spermatozoa assessed by cytofluorimetric analysis such as sperm viability (by using propidium iodide probe), acrosome reaction (by using PNA-FITC probe), sperm chromatin stability (by using acridine orange probe) basal ROS determination (by using DCFH-DA probe) and mitochondrial activity (by using JC-1 probe). The HeiferPlusTM treatment induced a significant (p<0.05) reduction of: sperm viability, basal ROS level and mitochondrial function, while sperm chromatin stability, measured as %-DNA fragmentation index (DFI), mean DFI and SD-DFI was not affected by the treatment. Concerning acrosome reaction, it was already observed a significant increase (p<0.05) of reacted live spermatozoa after 1,5 h incubation in either the presence or absence of Ca2+ in the semen treated with HeiferPlusTM compared to 0 h, while in the untreated semen the increase of reacted live spermatozoa was observed only after 3h incubation in presence of Ca2+. The treatment seems to affect some functional parameter of bull spermatozoa and to anticipate acrosome reaction. (literal)

Prodotto di

• Istituto di scienze delle produzioni alimentari (ISPA) (Istituto)
• Metodi innovativi per l'analisi e la riduzione di micotossine, funghi tossigeni ed allergeni nei prodotti agroalimentari (AG.P05.008.002) (Modulo)

Autore CNR

• FIORENZA MINERVINI (Unità di personale interno)
• Antonella Garbetta (Persona)

Incoming links:

Prodotto

• Istituto di scienze delle produzioni alimentari (ISPA) (Istituto)
• Metodi innovativi per l'analisi e la riduzione di micotossine, funghi tossigeni ed allergeni nei prodotti agroalimentari (AG.P05.008.002) (Modulo)

Autore CNR di

• FIORENZA MINERVINI (Unità di personale interno)
• Antonella Garbetta (Persona)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Reproduction in domestic animals (Rivista)