CBP/p300-mediated acetylation of PCNA is required for its chromatin removal and degradation in nucleotide excision repair (Abstract/Comunicazione in atti di convegno)

Type

• Abstract/Comunicazione in atti di convegno (Classe)
• Prodotto della ricerca (Classe)

Label

• CBP/p300-mediated acetylation of PCNA is required for its chromatin removal and degradation in nucleotide excision repair (Abstract/Comunicazione in atti di convegno) (literal)

Anno

• 2014-01-01T00:00:00+01:00 (literal)

Alternative label

• Tillhon M, Cazzalini O, Sommatis S, Dutto I, Bachi A, Rapp A, Nardo T, Scovassi AI, Necchi D, Cardoso MC, Stivala LA, Prosperi E (2014)
  CBP/p300-mediated acetylation of PCNA is required for its chromatin removal and degradation in nucleotide excision repair
  in XIII FISV Congress, Pisa, 24-27 Settembre 2014
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Tillhon M, Cazzalini O, Sommatis S, Dutto I, Bachi A, Rapp A, Nardo T, Scovassi AI, Necchi D, Cardoso MC, Stivala LA, Prosperi E (literal)

Pagina inizio

• 53 (literal)

Pagina fine

• 53 (literal)

Note

• Comunicazione (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Istituto di Genetica Molecolare CNR (IGM-CNR); Dipartimento di Medicina Molecolare, Università di Pavia; Istituto FIRC di Oncologia Molecolare, Milano; Technische Universität Darmstadt, Darmstadt, Germania; Dipartimento di Scienze del Farmaco, Università di Pavia. (literal)

Titolo

• CBP/p300-mediated acetylation of PCNA is required for its chromatin removal and degradation in nucleotide excision repair (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#isbn

• 978-88-6741-223-5 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#curatoriVolume

• FISV - Federazione Italiana Scienze della Vita (literal)

Abstract

• PCNA protein, a molecular platform recruiting and coordinating the activity of several DNA transaction factors, appears to be regulated by different post-translational modifications, among which acetylation. In this study, we have identified CBP, as another acetyltransferase (p300 homolog) interacting with PCNA, and found that PCNA is acetylated after UV-induced DNA damage. MS/MS analysis indicated that in vitro, CBP acetylates PCNA at residues Lys 13,14,77 and 80. Expression of PCNA mutated in these residues, resulted in impaired DNA replication and repair, enhanced sensitivity to UV radiation, and activated a DNA damage response. The same mutations prevented removal of chromatin-bound PCNA, and its subsequent proteolytic degradation after DNA damage. Depletion of CBP/p300 in normal fibroblasts prevented PCNA acetylation and degradation, while proteasome inhibition resulted in accumulation of acetylated PCNA. Our results suggest that PCNA acetylation by CBP and p300 is required to link DNA repair synthesis with chromatin removal and degradation of PCNA after DNA damage, to avoid its excessive accumulation, dangerous for genome stability. (literal)

Editore

• Pisa University Press (Editore)

Prodotto di

• Integrità genomica e checkpoints del ciclo cellulare (ME.P05.005.003) (Modulo)
• Institute of molecular genetics (IGM) (Istituto)

Autore CNR

• Ilaria DUTTO (Persona)
• Micol TILLHON (Unità di personale esterno)
• TIZIANA NARDO (Persona)
• ENNIO PROSPERI (Persona)
• ANNA SCOVASSI (Unità di personale interno)

Incoming links:

Prodotto

• Institute of molecular genetics (IGM) (Istituto)
• Integrità genomica e checkpoints del ciclo cellulare (ME.P05.005.003) (Modulo)

Autore CNR di

• ANNA SCOVASSI (Unità di personale interno)
• ENNIO PROSPERI (Persona)
• TIZIANA NARDO (Persona)
• Ilaria DUTTO (Persona)
• Micol TILLHON (Unità di personale esterno)

Editore di

• Pisa University Press (Editore)