http://www.cnr.it/ontology/cnr/individuo/prodotto/ID301373
D- and L-amino acids in Antarctic lakes: assessment of a very sensitive HPLC-MS method (Articolo in rivista)
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- Label
- D- and L-amino acids in Antarctic lakes: assessment of a very sensitive HPLC-MS method (Articolo in rivista) (literal)
- Anno
- 2014-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1007/s00216-014-7961-y (literal)
- Alternative label
Barbaro, Elena; Zangrando, Roberta; Vecchiato, Marco; Turetta, Clara; Barbante, Carlo; Gambaro, Andrea (2014)
D- and L-amino acids in Antarctic lakes: assessment of a very sensitive HPLC-MS method
in Analytical & bioanalytical chemistry (Print)
(literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Barbaro, Elena; Zangrando, Roberta; Vecchiato, Marco; Turetta, Clara; Barbante, Carlo; Gambaro, Andrea (literal)
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- ISI Web of Science (WOS) (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- Universita Ca Foscari Venezia; Consiglio Nazionale delle Ricerche (CNR); University of Siena (literal)
- Titolo
- D- and L-amino acids in Antarctic lakes: assessment of a very sensitive HPLC-MS method (literal)
- Abstract
- Amino acids represent a fraction of organic matter in marine and freshwater ecosystems, and a source of carbon, nitrogen and energy. l-Amino acids are the most common enantiomers in nature because these chiral forms are used during the biosynthesis of proteins and peptide. To the contrary, the occurrence of d-amino acids is usually linked to the presence of bacteria. We investigated the distribution of l- and d-amino acids in the lacustrine environment of Terra Nova Bay, Antarctica, in order to define their natural composition in this area and to individuate a possible relationship with primary production. A simultaneous chromatographic separation of 40 l- and d-amino acids was performed using a chiral stationary phase based on teicoplainin aglycone (chirobiotic tag). The chromatographic separation was coupled to two different mass spectrometers-an LTQ-Orbitrap XL (Thermo Fisher Scientific) and an API 4000 (ABSciex)-in order to investigate their quantitative performance. High-performance liquid chromatography coupled with mass spectrometry methods were evaluated through the estimation of their linear ranges, repeatability, accuracy and detection and quantification limits. The high-resolution mass spectrometer LTQ-Orbitrap XL presented detection limits between 0.4 and 7 mu g l (-1), while the triple quadrupole mass spectrometer API 4000 achieved the best detection limits reported in the literature for the quantification of amino acids (between 4 and 200 ng l (-1)). The most sensitive method, HPLC-API 4000, was applied to lake water samples. (literal)
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