http://www.cnr.it/ontology/cnr/individuo/prodotto/ID300300
An in vitro model based on human primary monocytes for evaluating the effects of engineered nanoparticles on the course of an inflammatory defence reaction. (Abstract/Poster in convegno)
- Type
- Label
- An in vitro model based on human primary monocytes for evaluating the effects of engineered nanoparticles on the course of an inflammatory defence reaction. (Abstract/Poster in convegno) (literal)
- Anno
- 2014-01-01T00:00:00+01:00 (literal)
- Alternative label
Li, Y., P. Italiani, E. Casals, V.F. Puntes, and D. Boraschi. (2014)
An in vitro model based on human primary monocytes for evaluating the effects of engineered nanoparticles on the course of an inflammatory defence reaction.
in NANOTOX 2014, 7th International Congress of Nanotoxicology, Antalya (Turkey), April 23-26, 2014
(literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Li, Y., P. Italiani, E. Casals, V.F. Puntes, and D. Boraschi. (literal)
- Note
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- 1Laboratory of Innate Immunity and Cytokines, Institute of Protein Biochemistry, National Research Council,
Napoli, Italy
2Institut Català de Nanotecnologia, Bellaterra, Spain (literal)
- Titolo
- An in vitro model based on human primary monocytes for evaluating the effects of engineered nanoparticles on the course of an inflammatory defence reaction. (literal)
- Abstract
- The possibility that engineered nanoparticles, even in the absence of direct toxicity, may interfere with the
normal development of the innate defensive response may cause inadequate immunity and eventual
pathological consequences.
The possible interference of nanoparticles with the normal development of the innate/inflammatory response
was evaluated with an in vitro model based on human primary monocytes, which reliably represents the human
inflammatory response. The course of the physiological inflammatory reaction, from initiation and development
to eventual resolution, was reproduced by exposing CD14+ human blood monocytes to a sequence of different
microenvironmental conditions (CCL2, LPS, TNF?, IFN-?, 37°C vs. 39°C). A parallel culture model was set up,
by varying the culture conditions, to mimic persistent pre-pathological inflammation.
The effect of endotoxin-free Au nanoparticles (diameter 10 nm, 1.28 ?g/ml, 0.16 cm2/1000000 cells) was
assessed on the course of the in vitro inflammatory reaction models. Gene expression and protein production
were analysed at different time points for the inflammatory cytokines and receptors IL-1?, IL-1?, IL-1RI, IL-6,
IL-18, IL-36? and CCL5, and for the anti-inflammatory factors IL-1Ra, IL-1RII and IL-18BP.
Preliminary results show that Au nanoparticles are unable to affect in a significant fashion the course of either
the physiological or the persistent inflammatory reaction in the in vitro monocyte-based models, suggesting
that these nanoparticles do not alter the innate defensive ability of the human body.
We conclude that the use of a valid and representative in vitro model of human inflammation may allow us to
realistically investigating the effects of nanoparticles on inflammatory/innate immune responses, thereby being
useful to predicting the immunological risk posed by engineered nanomaterials. (literal)
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