Amyloid-like fibrils in elastin-related polypeptides: Structural characterization and elastic properties (Articolo in rivista)

Type
Label
  • Amyloid-like fibrils in elastin-related polypeptides: Structural characterization and elastic properties (Articolo in rivista) (literal)
Anno
  • 2008-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1021/bm7010104 (literal)
Alternative label
  • del Mercato, LL; Maruccio, G; Pompa, PP; Bochicchio, B; Tamburro, AM; Cingolani, R; Rinaldi, R (2008)
    Amyloid-like fibrils in elastin-related polypeptides: Structural characterization and elastic properties
    in Biomacromolecules
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • del Mercato, LL; Maruccio, G; Pompa, PP; Bochicchio, B; Tamburro, AM; Cingolani, R; Rinaldi, R (literal)
Pagina inizio
  • 796 (literal)
Pagina fine
  • 803 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 9 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 3 (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • \"[del Mercato, Loretta L.; Maruccio, Giuseppe; Pompa, Pier Paolo; Cingolani, Roberto; Rinaldi, Ross] Univ Salento, ISUFI, IIT Res Unit, Natl Nanotechnol Lab INFM CNR, I-73100 Lecce, Italy; [Lochicchio, Brigida; Tamburro, Antonio M.] Univ Basilicata, Dept Chem, I-85100 Potenza, Italy (literal)
Titolo
  • Amyloid-like fibrils in elastin-related polypeptides: Structural characterization and elastic properties (literal)
Abstract
  • We report on the structural characterization of amyloid-like fibrils, self-assembled from synthetic polypentapeptides poly(ValGlyGlyLeuGly), whose monomeric sequence is a recurring, simple building block of elastin. This polymer adopts a P-sheet structure as revealed by circular dichroism and Fourier transform infrared spectroscopy. Furthermore, Thioflavin-T and Congo red bireftingence assays confirm the presence of amyloid-like structures. To analyze the supramolecular assembly and elastic properties of the fibrils, we employed atomic force microsocopy and spectroscopy, measuring also the elasticity of mature elastin for a comparative analysis. In the case of fibrils we estimated a Young's modulus ranging from 3.5 to 7 MPa, whereas for elastin it is around I MPa. The possibility to section individual fibrils with nanometric control by the AFM tip, realizing biomolecular gaps in the 100 nm range, is also demonstrated. These results are expected to open interesting perspectives for the fabrication of protein-inspired nanostructures with specific physical and chemical properties for applications in biotechnology and tissue engineering. (literal)
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